gene: At4g16160

insert: T-DNA_LB.SALK_076407.51.85.x

[Graphical View]
Sequence
GTGGAGGAGCTCATGATTGGGTTAGTCTAATTAATTTGACTTGTGTCATTTTGTTTTCATCAACGAACAAATGATGAACATGATCTTTAATTAGTATGTA
ATTTGATATGTGTCACAGCGGAACAGCGCGGTAGCTGGAGCATTGACAGGAGCGGCAATGGCTATGACGACGTCTGAGAGGACAAGCCATGAGCAAGTGG
TTCAGTCTGCTCTCACTGGAGCAGCTATTTCCACCGCTGCTAATCTCCTTTCTAGCGTTTTCTAGATGATTTGTATTAGAAGCTTTAATGCATAAGGGTT
GTTTCATGTATCAGTTATGTTTCTTTTTGTGAAAACGAATTCGCTAAAGGTCTACTAAGATCTTTTCCTGAAACTCTCGAAGCTGGAGAGAGATCGTTTT
GTTATCTGTTTGGAAAATATTTGCACTATATATATCTTGAGTAGTACCTATTACGTAACCTTATCTATTTGGATAAAAATGCGGGTCTTGTGGGCTCGCT
GCACAGACAATAACCCGTGAGGCAGCGTAACAACAAAAACGACATTCGTGTTGGAAATCAAAATGGTGACGCTCATGTGAAGGTGAATAATATTTGTCAT
TG
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 164
PRIMER_PAIR_PENALTY 0.1292
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 55.000
PRIMER_LEFT_POSITION 222 PRIMER_RIGHT_POSITION 385
PRIMER_LEFT_PENALTY 0.003166 PRIMER_RIGHT_PENALTY 0.126032
PRIMER_LEFT_SELF_END 2.00 PRIMER_RIGHT_SELF_END 1.00
PRIMER_LEFT_SEQUENCE AGCTATTTCCACCGCTGCTA PRIMER_RIGHT_SEQUENCE CCAGCTTCGAGAGTTTCAGG
PRIMER_LEFT_END_STABILITY 7.5000 PRIMER_RIGHT_END_STABILITY 8.2000
PRIMER_LEFT_TM 60.003 PRIMER_RIGHT_TM 60.126
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 218
PRIMER_PAIR_PENALTY_1 0.1330
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.993 PRIMER_RIGHT_1_TM 60.126
PRIMER_LEFT_1_END_STABILITY 9.7000 PRIMER_RIGHT_1_END_STABILITY 8.2000
PRIMER_LEFT_1_GC_PERCENT 60.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE GACGTCTGAGAGGACAAGCC PRIMER_RIGHT_1_SEQUENCE CCAGCTTCGAGAGTTTCAGG
PRIMER_LEFT_1_SELF_ANY 6.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 168,20 PRIMER_RIGHT_1 385,20
PRIMER_LEFT_1_SELF_END 1.00 PRIMER_RIGHT_1_SELF_END 1.00
PRIMER_LEFT_1_PENALTY 0.006937 PRIMER_RIGHT_1_PENALTY 0.126032


Oligo 3
PRIMER_PRODUCT_SIZE_2 291
PRIMER_PAIR_2_COMPL_ANY 6.00
PRIMER_PAIR_2_COMPL_END 3.00
PRIMER_PAIR_PENALTY_2 0.1684
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 7.5000 PRIMER_RIGHT_2_END_STABILITY 9.9000
PRIMER_LEFT_2_SEQUENCE AGCTATTTCCACCGCTGCTA PRIMER_RIGHT_2_SEQUENCE TTATTGTCTGTGCAGCGAGC
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 222,20 PRIMER_RIGHT_2 512,20
PRIMER_LEFT_2_TM 60.003 PRIMER_RIGHT_2_TM 60.165
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.003166 PRIMER_RIGHT_2_PENALTY 0.165200


Oligo 4
PRIMER_PRODUCT_SIZE_3 215
PRIMER_PAIR_PENALTY_3 0.2463
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.126032 PRIMER_RIGHT_3_PENALTY 0.120245
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 1.00
PRIMER_LEFT_3_END_STABILITY 9.7000 PRIMER_RIGHT_3_END_STABILITY 8.4000
PRIMER_LEFT_3_GC_PERCENT 55.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 60.126 PRIMER_RIGHT_3_TM 60.120
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE CCTGAAACTCTCGAAGCTGG PRIMER_RIGHT_3_SEQUENCE TTCACATGAGCGTCACCATT
PRIMER_LEFT_3 366,20 PRIMER_RIGHT_3 580,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 214
PRIMER_PAIR_4_COMPL_END 2.00
PRIMER_PAIR_PENALTY_4 0.2463
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CCTGAAACTCTCGAAGCTGG PRIMER_RIGHT_4_SEQUENCE TCACATGAGCGTCACCATTT
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 3.00
PRIMER_LEFT_4_TM 60.126 PRIMER_RIGHT_4_TM 60.120
PRIMER_LEFT_4_PENALTY 0.126032 PRIMER_RIGHT_4_PENALTY 0.120245
PRIMER_LEFT_4 366,20 PRIMER_RIGHT_4 579,20
PRIMER_LEFT_4_END_STABILITY 9.7000 PRIMER_RIGHT_4_END_STABILITY 7.2000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00



insert: T-DNA_LB.SALK_076409.48.80.x

[Graphical View]
Sequence
GTGGAGGAGCTCATGATTGGGTTAGTCTAATTAATTTGACTTGTGTCATTTTGTTTTCATCAACGAACAAATGATGAACATGATCTTTAATTAGTATGTA
ATTTGATATGTGTCACAGCGGAACAGCGCGGTAGCTGGAGCATTGACAGGAGCGGCAATGGCTATGACGACGTCTGAGAGGACAAGCCATGAGCAAGTGG
TTCAGTCTGCTCTCACTGGAGCAGCTATTTCCACCGCTGCTAATCTCCTTTCTAGCGTTTTCTAGATGATTTGTATTAGAAGCTTTAATGCATAAGGGTT
GTTTCATGTATCAGTTATGTTTCTTTTTGTGAAAACGAATTCGCTAAAGGTCTACTAAGATCTTTTCCTGAAACTCTCGAAGCTGGAGAGAGATCGTTTT
GTTATCTGTTTGGAAAATATTTGCACTATATATATCTTGAGTAGTACCTATTACGTAACCTTATCTATTTGGATAAAAATGCGGGTCTTGTGGGCTCGCT
GCACAGACAATAACCCGTGAGGCAGCGTAACAACAAAAACGACATTCGTGTTGGAAATCAAAATGGTGACGCTCATGTGAAGGTGAATAATATTTGTCAT
TG
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 164
PRIMER_PAIR_PENALTY 0.1292
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 55.000
PRIMER_LEFT_POSITION 222 PRIMER_RIGHT_POSITION 385
PRIMER_LEFT_PENALTY 0.003166 PRIMER_RIGHT_PENALTY 0.126032
PRIMER_LEFT_SELF_END 2.00 PRIMER_RIGHT_SELF_END 1.00
PRIMER_LEFT_SEQUENCE AGCTATTTCCACCGCTGCTA PRIMER_RIGHT_SEQUENCE CCAGCTTCGAGAGTTTCAGG
PRIMER_LEFT_END_STABILITY 7.5000 PRIMER_RIGHT_END_STABILITY 8.2000
PRIMER_LEFT_TM 60.003 PRIMER_RIGHT_TM 60.126
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 218
PRIMER_PAIR_PENALTY_1 0.1330
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.993 PRIMER_RIGHT_1_TM 60.126
PRIMER_LEFT_1_END_STABILITY 9.7000 PRIMER_RIGHT_1_END_STABILITY 8.2000
PRIMER_LEFT_1_GC_PERCENT 60.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE GACGTCTGAGAGGACAAGCC PRIMER_RIGHT_1_SEQUENCE CCAGCTTCGAGAGTTTCAGG
PRIMER_LEFT_1_SELF_ANY 6.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 168,20 PRIMER_RIGHT_1 385,20
PRIMER_LEFT_1_SELF_END 1.00 PRIMER_RIGHT_1_SELF_END 1.00
PRIMER_LEFT_1_PENALTY 0.006937 PRIMER_RIGHT_1_PENALTY 0.126032


Oligo 3
PRIMER_PRODUCT_SIZE_2 291
PRIMER_PAIR_2_COMPL_ANY 6.00
PRIMER_PAIR_2_COMPL_END 3.00
PRIMER_PAIR_PENALTY_2 0.1684
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 7.5000 PRIMER_RIGHT_2_END_STABILITY 9.9000
PRIMER_LEFT_2_SEQUENCE AGCTATTTCCACCGCTGCTA PRIMER_RIGHT_2_SEQUENCE TTATTGTCTGTGCAGCGAGC
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 222,20 PRIMER_RIGHT_2 512,20
PRIMER_LEFT_2_TM 60.003 PRIMER_RIGHT_2_TM 60.165
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.003166 PRIMER_RIGHT_2_PENALTY 0.165200


Oligo 4
PRIMER_PRODUCT_SIZE_3 215
PRIMER_PAIR_PENALTY_3 0.2463
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.126032 PRIMER_RIGHT_3_PENALTY 0.120245
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 1.00
PRIMER_LEFT_3_END_STABILITY 9.7000 PRIMER_RIGHT_3_END_STABILITY 8.4000
PRIMER_LEFT_3_GC_PERCENT 55.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 60.126 PRIMER_RIGHT_3_TM 60.120
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE CCTGAAACTCTCGAAGCTGG PRIMER_RIGHT_3_SEQUENCE TTCACATGAGCGTCACCATT
PRIMER_LEFT_3 366,20 PRIMER_RIGHT_3 580,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 214
PRIMER_PAIR_4_COMPL_END 2.00
PRIMER_PAIR_PENALTY_4 0.2463
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CCTGAAACTCTCGAAGCTGG PRIMER_RIGHT_4_SEQUENCE TCACATGAGCGTCACCATTT
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 3.00
PRIMER_LEFT_4_TM 60.126 PRIMER_RIGHT_4_TM 60.120
PRIMER_LEFT_4_PENALTY 0.126032 PRIMER_RIGHT_4_PENALTY 0.120245
PRIMER_LEFT_4 366,20 PRIMER_RIGHT_4 579,20
PRIMER_LEFT_4_END_STABILITY 9.7000 PRIMER_RIGHT_4_END_STABILITY 7.2000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00



insert: T-DNA_LB.SALK_014191

[Graphical View]
Sequence
TCGTGGAGGAGCTCATGATTGGGTTAGTCTAATTAATTTGACTTGTGTCATTTTGTTTTCATCAACGAACAAATGATGAACATGATCTTTAATTAGTATG
TAATTTGATATGTGTCACAGCGGAACAGCGCGGTAGCTGGAGCATTGACAGGAGCGGCAATGGCTATGACGACGTCTGAGAGGACAAGCCATGAGCAAGT
GGTTCAGTCTGCTCTCACTGGAGCAGCTATTTCCACCGCTGCTAATCTCCTTTCTAGCGTTTTCTAGATGATTTGTATTAGAAGCTTTAATGCATAAGGG
TTGTTTCATGTATCAGTTATGTTTCTTTTTGTGAAAACGAATTCGCTAAAGGTCTACTAAGATCTTTTCCTGAAACTCTCGAAGCTGGAGAGAGATCGTT
TTGTTATCTGTTTGGAAAATATTTGCACTATATATATCTTGAGTAGTACCTATTACGTAACCTTATCTATTTGGATAAAAATGCGGGTCTTGTGGGCTCG
CTGCACAGACAATAACCCGTGAGGCAGCGTAACAACAAAAACGACATTCGTGTTGGAAATCAAAATGGTGACGCTCATGTGAAGGTGAATAATATTTGTC
AT
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 164
PRIMER_PAIR_PENALTY 0.1292
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 55.000
PRIMER_LEFT_POSITION 224 PRIMER_RIGHT_POSITION 387
PRIMER_LEFT_PENALTY 0.003166 PRIMER_RIGHT_PENALTY 0.126032
PRIMER_LEFT_SELF_END 2.00 PRIMER_RIGHT_SELF_END 1.00
PRIMER_LEFT_SEQUENCE AGCTATTTCCACCGCTGCTA PRIMER_RIGHT_SEQUENCE CCAGCTTCGAGAGTTTCAGG
PRIMER_LEFT_END_STABILITY 7.5000 PRIMER_RIGHT_END_STABILITY 8.2000
PRIMER_LEFT_TM 60.003 PRIMER_RIGHT_TM 60.126
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 218
PRIMER_PAIR_PENALTY_1 0.1330
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.993 PRIMER_RIGHT_1_TM 60.126
PRIMER_LEFT_1_END_STABILITY 9.7000 PRIMER_RIGHT_1_END_STABILITY 8.2000
PRIMER_LEFT_1_GC_PERCENT 60.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE GACGTCTGAGAGGACAAGCC PRIMER_RIGHT_1_SEQUENCE CCAGCTTCGAGAGTTTCAGG
PRIMER_LEFT_1_SELF_ANY 6.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 170,20 PRIMER_RIGHT_1 387,20
PRIMER_LEFT_1_SELF_END 1.00 PRIMER_RIGHT_1_SELF_END 1.00
PRIMER_LEFT_1_PENALTY 0.006937 PRIMER_RIGHT_1_PENALTY 0.126032


Oligo 3
PRIMER_PRODUCT_SIZE_2 291
PRIMER_PAIR_2_COMPL_ANY 6.00
PRIMER_PAIR_2_COMPL_END 3.00
PRIMER_PAIR_PENALTY_2 0.1684
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 7.5000 PRIMER_RIGHT_2_END_STABILITY 9.9000
PRIMER_LEFT_2_SEQUENCE AGCTATTTCCACCGCTGCTA PRIMER_RIGHT_2_SEQUENCE TTATTGTCTGTGCAGCGAGC
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 224,20 PRIMER_RIGHT_2 514,20
PRIMER_LEFT_2_TM 60.003 PRIMER_RIGHT_2_TM 60.165
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.003166 PRIMER_RIGHT_2_PENALTY 0.165200


Oligo 4
PRIMER_PRODUCT_SIZE_3 215
PRIMER_PAIR_PENALTY_3 0.2463
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.126032 PRIMER_RIGHT_3_PENALTY 0.120245
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 1.00
PRIMER_LEFT_3_END_STABILITY 9.7000 PRIMER_RIGHT_3_END_STABILITY 8.4000
PRIMER_LEFT_3_GC_PERCENT 55.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 60.126 PRIMER_RIGHT_3_TM 60.120
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE CCTGAAACTCTCGAAGCTGG PRIMER_RIGHT_3_SEQUENCE TTCACATGAGCGTCACCATT
PRIMER_LEFT_3 368,20 PRIMER_RIGHT_3 582,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 214
PRIMER_PAIR_4_COMPL_END 2.00
PRIMER_PAIR_PENALTY_4 0.2463
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CCTGAAACTCTCGAAGCTGG PRIMER_RIGHT_4_SEQUENCE TCACATGAGCGTCACCATTT
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 3.00
PRIMER_LEFT_4_TM 60.126 PRIMER_RIGHT_4_TM 60.120
PRIMER_LEFT_4_PENALTY 0.126032 PRIMER_RIGHT_4_PENALTY 0.120245
PRIMER_LEFT_4 368,20 PRIMER_RIGHT_4 581,20
PRIMER_LEFT_4_END_STABILITY 9.7000 PRIMER_RIGHT_4_END_STABILITY 7.2000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00