gene: At3g62880

insert: T-DNA_LB.GK-830H01-025745

[Graphical View]
Sequence
GCAATCACTACTTGAAGAATTTAGATCTAAGTTAAAGAAGAGCAGAGCAACTAATCCAAAAAAAATTACAACTAAAAGAAATCTGCATTATATATTATTA
CATGGCAAGCAAAACAGAGAATTATGATCTTTCAAAACAAAAAAAAAGTATATGCAGCATTCTTCTTCTAGTGACAAAGAAGAGAATTATCTTAAGTCCA
AAATTACAGATTTATACCCAAATTCCATAGTCATTGAAGATTAATTTGGGTGGAAAAACAATAGAGGAAGCAAATTACCGCATTGAAATCCGAATCTGCC
AATGGATTTCGCCACAAAGGAAGCCTGAGAAACGCCGCTTAAACCTGGCGAAGCAAATCAGCAACAAAGCACAGATTAATGAATTGAAAAGTGTAAAAAC
TCTCTAAATCAAAGCTAATTTCAATACCTATTTTACGAGCGTCACGAGGTCCGGCGCATAACCCATATAATCCACCGGCCTGAGCTTCAACAAAAATCTC
AGACATCTATCTATACACACACTAACACAAACATGAATTTCAATTGGATACTAGTGATTACCGTCGCGACACGGAGAACTGACTCGACGGTTAGGGAAGA
GC
Oligo 1
PRIMER_PAIR_COMPL_ANY 7.00
PRIMER_PRODUCT_SIZE 182
PRIMER_PAIR_PENALTY 0.1669
PRIMER_PAIR_COMPL_END 3.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 45.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 100 PRIMER_RIGHT_POSITION 281
PRIMER_LEFT_PENALTY 0.012060 PRIMER_RIGHT_PENALTY 0.154878
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_SEQUENCE TGCGGTAATTTGCTTCCTCT
PRIMER_LEFT_END_STABILITY 6.4000 PRIMER_RIGHT_END_STABILITY 7.9000
PRIMER_LEFT_TM 59.988 PRIMER_RIGHT_TM 59.845
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 251
PRIMER_PAIR_PENALTY_1 0.2548
PRIMER_PAIR_1_COMPL_END 0.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.992 PRIMER_RIGHT_1_TM 60.246
PRIMER_LEFT_1_END_STABILITY 7.0000 PRIMER_RIGHT_1_END_STABILITY 6.4000
PRIMER_LEFT_1_GC_PERCENT 40.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE CGAAGCAAATCAGCAACAAA PRIMER_RIGHT_1_SEQUENCE CTTCCCTAACCGTCGAGTCA
PRIMER_LEFT_1_SELF_ANY 2.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 348,20 PRIMER_RIGHT_1 598,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 3.00
PRIMER_LEFT_1_PENALTY 0.008354 PRIMER_RIGHT_1_PENALTY 0.246438


Oligo 3
PRIMER_PRODUCT_SIZE_2 285
PRIMER_PAIR_2_COMPL_ANY 5.00
PRIMER_PAIR_2_COMPL_END 1.00
PRIMER_PAIR_PENALTY_2 0.2603
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 8.7000 PRIMER_RIGHT_2_END_STABILITY 6.4000
PRIMER_LEFT_2_SEQUENCE CAAAGGAAGCCTGAGAAACG PRIMER_RIGHT_2_SEQUENCE CTTCCCTAACCGTCGAGTCA
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 3.00
PRIMER_LEFT_2 314,20 PRIMER_RIGHT_2 598,20
PRIMER_LEFT_2_TM 59.986 PRIMER_RIGHT_2_TM 60.246
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 3.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.013872 PRIMER_RIGHT_2_PENALTY 0.246438


Oligo 4
PRIMER_PRODUCT_SIZE_3 101
PRIMER_PAIR_PENALTY_3 0.2787
PRIMER_PAIR_3_COMPL_ANY 3.00
PRIMER_PAIR_3_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.008354 PRIMER_RIGHT_3_PENALTY 0.270338
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 2.00
PRIMER_LEFT_3_END_STABILITY 7.0000 PRIMER_RIGHT_3_END_STABILITY 7.2000
PRIMER_LEFT_3_GC_PERCENT 40.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 59.992 PRIMER_RIGHT_3_TM 60.270
PRIMER_LEFT_3_SELF_ANY 2.00 PRIMER_RIGHT_3_SELF_ANY 5.00
PRIMER_LEFT_3_SEQUENCE CGAAGCAAATCAGCAACAAA PRIMER_RIGHT_3_SEQUENCE CCTCGTGACGCTCGTAAAAT
PRIMER_LEFT_3 348,20 PRIMER_RIGHT_3 448,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE_4 135
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.2842
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CAAAGGAAGCCTGAGAAACG PRIMER_RIGHT_4_SEQUENCE CCTCGTGACGCTCGTAAAAT
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 2.00
PRIMER_LEFT_4_TM 59.986 PRIMER_RIGHT_4_TM 60.270
PRIMER_LEFT_4_PENALTY 0.013872 PRIMER_RIGHT_4_PENALTY 0.270338
PRIMER_LEFT_4 314,20 PRIMER_RIGHT_4 448,20
PRIMER_LEFT_4_END_STABILITY 8.7000 PRIMER_RIGHT_4_END_STABILITY 7.2000
PRIMER_LEFT_4_GC_PERCENT 50.000 PRIMER_RIGHT_4_GC_PERCENT 50.000
PRIMER_LEFT_4_SELF_ANY 3.00 PRIMER_RIGHT_4_SELF_ANY 5.00



insert: T-DNA_LB.GK-830H01-025706

[Graphical View]
Sequence
AAAGAAGCAATCACTACTTGAAGAATTTAGATCTAAGTTAAAGAAGAGCAGAGCAACTAATCCAAAAAAAATTACAACTAAAAGAAATCTGCATTATATA
TTATTACATGGCAAGCAAAACAGAGAATTATGATCTTTCAAAACAAAAAAAAAGTATATGCAGCATTCTTCTTCTAGTGACAAAGAAGAGAATTATCTTA
AGTCCAAAATTACAGATTTATACCCAAATTCCATAGTCATTGAAGATTAATTTGGGTGGAAAAACAATAGAGGAAGCAAATTACCGCATTGAAATCCGAA
TCTGCCAATGGATTTCGCCACAAAGGAAGCCTGAGAAACGCCGCTTAAACCTGGCGAAGCAAATCAGCAACAAAGCACAGATTAATGAATTGAAAAGTGT
AAAAACTCTCTAAATCAAAGCTAATTTCAATACCTATTTTACGAGCGTCACGAGGTCCGGCGCATAACCCATATAATCCACCGGCCTGAGCTTCAACAAA
AATCTCAGACATCTATCTATACACACACTAACACAAACATGAATTTCAATTGGATACTAGTGATTACCGTCGCGACACGGAGAACTGACTCGACGGTTAG
GG
Oligo 1
PRIMER_PAIR_COMPL_ANY 7.00
PRIMER_PRODUCT_SIZE 182
PRIMER_PAIR_PENALTY 0.1669
PRIMER_PAIR_COMPL_END 3.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 45.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 106 PRIMER_RIGHT_POSITION 287
PRIMER_LEFT_PENALTY 0.012060 PRIMER_RIGHT_PENALTY 0.154878
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_SEQUENCE TGCGGTAATTTGCTTCCTCT
PRIMER_LEFT_END_STABILITY 6.4000 PRIMER_RIGHT_END_STABILITY 7.9000
PRIMER_LEFT_TM 59.988 PRIMER_RIGHT_TM 59.845
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 101
PRIMER_PAIR_PENALTY_1 0.2787
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 3.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.992 PRIMER_RIGHT_1_TM 60.270
PRIMER_LEFT_1_END_STABILITY 7.0000 PRIMER_RIGHT_1_END_STABILITY 7.2000
PRIMER_LEFT_1_GC_PERCENT 40.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE CGAAGCAAATCAGCAACAAA PRIMER_RIGHT_1_SEQUENCE CCTCGTGACGCTCGTAAAAT
PRIMER_LEFT_1_SELF_ANY 2.00 PRIMER_RIGHT_1_SELF_ANY 5.00
PRIMER_LEFT_1 354,20 PRIMER_RIGHT_1 454,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.008354 PRIMER_RIGHT_1_PENALTY 0.270338


Oligo 3
PRIMER_PRODUCT_SIZE_2 135
PRIMER_PAIR_2_COMPL_ANY 3.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.2842
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 8.7000 PRIMER_RIGHT_2_END_STABILITY 7.2000
PRIMER_LEFT_2_SEQUENCE CAAAGGAAGCCTGAGAAACG PRIMER_RIGHT_2_SEQUENCE CCTCGTGACGCTCGTAAAAT
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 320,20 PRIMER_RIGHT_2 454,20
PRIMER_LEFT_2_TM 59.986 PRIMER_RIGHT_2_TM 60.270
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 3.00 PRIMER_RIGHT_2_SELF_ANY 5.00
PRIMER_LEFT_2_PENALTY 0.013872 PRIMER_RIGHT_2_PENALTY 0.270338


Oligo 4
PRIMER_PRODUCT_SIZE_3 223
PRIMER_PAIR_PENALTY_3 0.2879
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.008354 PRIMER_RIGHT_3_PENALTY 0.279536
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 3.00
PRIMER_LEFT_3_END_STABILITY 7.0000 PRIMER_RIGHT_3_END_STABILITY 5.7000
PRIMER_LEFT_3_GC_PERCENT 40.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 59.992 PRIMER_RIGHT_3_TM 60.280
PRIMER_LEFT_3_SELF_ANY 2.00 PRIMER_RIGHT_3_SELF_ANY 8.00
PRIMER_LEFT_3_SEQUENCE CGAAGCAAATCAGCAACAAA PRIMER_RIGHT_3_SEQUENCE TGTCGCGACGGTAATCACTA
PRIMER_LEFT_3 354,20 PRIMER_RIGHT_3 576,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE_4 257
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.2934
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CAAAGGAAGCCTGAGAAACG PRIMER_RIGHT_4_SEQUENCE TGTCGCGACGGTAATCACTA
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 3.00
PRIMER_LEFT_4_TM 59.986 PRIMER_RIGHT_4_TM 60.280
PRIMER_LEFT_4_PENALTY 0.013872 PRIMER_RIGHT_4_PENALTY 0.279536
PRIMER_LEFT_4 320,20 PRIMER_RIGHT_4 576,20
PRIMER_LEFT_4_END_STABILITY 8.7000 PRIMER_RIGHT_4_END_STABILITY 5.7000
PRIMER_LEFT_4_GC_PERCENT 50.000 PRIMER_RIGHT_4_GC_PERCENT 50.000
PRIMER_LEFT_4_SELF_ANY 3.00 PRIMER_RIGHT_4_SELF_ANY 8.00



insert: T-DNA_LB.SALK_109275.54.80.x

[Graphical View]
Sequence
AGCAGAGACGAGGCCAGCCATGCCAACAACCTGTGTCCAGTTTCTTGTGCTAATGGCAACAGCTGCTCCCGCCACAGCTCCTCCTACCAAAGCATTCACC
CAATCATTCTTGCCTCGATATCGTTGAAGCCCACAATGTGTCATAGTAAACACACCACTTACAAGACCTGAAACAAAAATGAATTCTTGATGCATATACC
AAAATCTAAAGAAGCAATCACTACTTGAAGAATTTAGATCTAAGTTAAAGAAGAGCAGAGCAACTAATCCAAAAAAAATTACAACTAAAAGAAATCTGCA
TTATATATTATTACATGGCAAGCAAAACAGAGAATTATGATCTTTCAAAACAAAAAAAAAGTATATGCAGCATTCTTCTTCTAGTGACAAAGAAGAGAAT
TATCTTAAGTCCAAAATTACAGATTTATACCCAAATTCCATAGTCATTGAAGATTAATTTGGGTGGAAAAACAATAGAGGAAGCAAATTACCGCATTGAA
ATCCGAATCTGCCAATGGATTTCGCCACAAAGGAAGCCTGAGAAACGCCGCTTAAACCTGGCGAAGCAAATCAGCAACAAAGCACAGATTAATGAATTGA
AA
Oligo 1
PRIMER_PAIR_COMPL_ANY 7.00
PRIMER_PRODUCT_SIZE 182
PRIMER_PAIR_PENALTY 0.1669
PRIMER_PAIR_COMPL_END 3.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 45.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 313 PRIMER_RIGHT_POSITION 494
PRIMER_LEFT_PENALTY 0.012060 PRIMER_RIGHT_PENALTY 0.154878
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_SEQUENCE TGCGGTAATTTGCTTCCTCT
PRIMER_LEFT_END_STABILITY 6.4000 PRIMER_RIGHT_END_STABILITY 7.9000
PRIMER_LEFT_TM 59.988 PRIMER_RIGHT_TM 59.845
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 193
PRIMER_PAIR_PENALTY_1 0.3010
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.988 PRIMER_RIGHT_1_TM 60.289
PRIMER_LEFT_1_END_STABILITY 6.4000 PRIMER_RIGHT_1_END_STABILITY 5.6000
PRIMER_LEFT_1_GC_PERCENT 45.000 PRIMER_RIGHT_1_GC_PERCENT 40.000
PRIMER_LEFT_1_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_1_SEQUENCE TCGGATTTCAATGCGGTAAT
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 6.00
PRIMER_LEFT_1 313,20 PRIMER_RIGHT_1 505,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.012060 PRIMER_RIGHT_1_PENALTY 0.288917


Oligo 3
PRIMER_PRODUCT_SIZE_2 195
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 1.00
PRIMER_PAIR_PENALTY_2 0.3010
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 6.4000 PRIMER_RIGHT_2_END_STABILITY 8.9000
PRIMER_LEFT_2_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_2_SEQUENCE ATTCGGATTTCAATGCGGTA
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 313,20 PRIMER_RIGHT_2 507,20
PRIMER_LEFT_2_TM 59.988 PRIMER_RIGHT_2_TM 60.289
PRIMER_LEFT_2_GC_PERCENT 45.000 PRIMER_RIGHT_2_GC_PERCENT 40.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 6.00
PRIMER_LEFT_2_PENALTY 0.012060 PRIMER_RIGHT_2_PENALTY 0.288917


Oligo 4
PRIMER_PRODUCT_SIZE_3 231
PRIMER_PAIR_PENALTY_3 0.3785
PRIMER_PAIR_3_COMPL_ANY 6.00
PRIMER_PAIR_3_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.012060 PRIMER_RIGHT_3_PENALTY 0.366450
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.4000 PRIMER_RIGHT_3_END_STABILITY 8.2000
PRIMER_LEFT_3_GC_PERCENT 45.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 59.988 PRIMER_RIGHT_3_TM 60.366
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 3.00
PRIMER_LEFT_3_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_3_SEQUENCE TTCTCAGGCTTCCTTTGTGG
PRIMER_LEFT_3 313,20 PRIMER_RIGHT_3 543,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 203
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 0.4261
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_4_SEQUENCE ATTGGCAGATTCGGATTTCA
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 1.00
PRIMER_LEFT_4_TM 59.988 PRIMER_RIGHT_4_TM 60.414
PRIMER_LEFT_4_PENALTY 0.012060 PRIMER_RIGHT_4_PENALTY 0.414033
PRIMER_LEFT_4 313,20 PRIMER_RIGHT_4 515,20
PRIMER_LEFT_4_END_STABILITY 6.4000 PRIMER_RIGHT_4_END_STABILITY 7.3000
PRIMER_LEFT_4_GC_PERCENT 45.000 PRIMER_RIGHT_4_GC_PERCENT 40.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00



insert: T-DNA_LB.SALK_109276.43.65.x

[Graphical View]
Sequence
AAGCAGAGACGAGGCCAGCCATGCCAACAACCTGTGTCCAGTTTCTTGTGCTAATGGCAACAGCTGCTCCCGCCACAGCTCCTCCTACCAAAGCATTCAC
CCAATCATTCTTGCCTCGATATCGTTGAAGCCCACAATGTGTCATAGTAAACACACCACTTACAAGACCTGAAACAAAAATGAATTCTTGATGCATATAC
CAAAATCTAAAGAAGCAATCACTACTTGAAGAATTTAGATCTAAGTTAAAGAAGAGCAGAGCAACTAATCCAAAAAAAATTACAACTAAAAGAAATCTGC
ATTATATATTATTACATGGCAAGCAAAACAGAGAATTATGATCTTTCAAAACAAAAAAAAAGTATATGCAGCATTCTTCTTCTAGTGACAAAGAAGAGAA
TTATCTTAAGTCCAAAATTACAGATTTATACCCAAATTCCATAGTCATTGAAGATTAATTTGGGTGGAAAAACAATAGAGGAAGCAAATTACCGCATTGA
AATCCGAATCTGCCAATGGATTTCGCCACAAAGGAAGCCTGAGAAACGCCGCTTAAACCTGGCGAAGCAAATCAGCAACAAAGCACAGATTAATGAATTG
AA
Oligo 1
PRIMER_PAIR_COMPL_ANY 7.00
PRIMER_PRODUCT_SIZE 182
PRIMER_PAIR_PENALTY 0.1669
PRIMER_PAIR_COMPL_END 3.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 45.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 314 PRIMER_RIGHT_POSITION 495
PRIMER_LEFT_PENALTY 0.012060 PRIMER_RIGHT_PENALTY 0.154878
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_SEQUENCE TGCGGTAATTTGCTTCCTCT
PRIMER_LEFT_END_STABILITY 6.4000 PRIMER_RIGHT_END_STABILITY 7.9000
PRIMER_LEFT_TM 59.988 PRIMER_RIGHT_TM 59.845
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 193
PRIMER_PAIR_PENALTY_1 0.3010
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.988 PRIMER_RIGHT_1_TM 60.289
PRIMER_LEFT_1_END_STABILITY 6.4000 PRIMER_RIGHT_1_END_STABILITY 5.6000
PRIMER_LEFT_1_GC_PERCENT 45.000 PRIMER_RIGHT_1_GC_PERCENT 40.000
PRIMER_LEFT_1_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_1_SEQUENCE TCGGATTTCAATGCGGTAAT
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 6.00
PRIMER_LEFT_1 314,20 PRIMER_RIGHT_1 506,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.012060 PRIMER_RIGHT_1_PENALTY 0.288917


Oligo 3
PRIMER_PRODUCT_SIZE_2 195
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 1.00
PRIMER_PAIR_PENALTY_2 0.3010
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 6.4000 PRIMER_RIGHT_2_END_STABILITY 8.9000
PRIMER_LEFT_2_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_2_SEQUENCE ATTCGGATTTCAATGCGGTA
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 314,20 PRIMER_RIGHT_2 508,20
PRIMER_LEFT_2_TM 59.988 PRIMER_RIGHT_2_TM 60.289
PRIMER_LEFT_2_GC_PERCENT 45.000 PRIMER_RIGHT_2_GC_PERCENT 40.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 6.00
PRIMER_LEFT_2_PENALTY 0.012060 PRIMER_RIGHT_2_PENALTY 0.288917


Oligo 4
PRIMER_PRODUCT_SIZE_3 231
PRIMER_PAIR_PENALTY_3 0.3785
PRIMER_PAIR_3_COMPL_ANY 6.00
PRIMER_PAIR_3_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.012060 PRIMER_RIGHT_3_PENALTY 0.366450
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.4000 PRIMER_RIGHT_3_END_STABILITY 8.2000
PRIMER_LEFT_3_GC_PERCENT 45.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 59.988 PRIMER_RIGHT_3_TM 60.366
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 3.00
PRIMER_LEFT_3_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_3_SEQUENCE TTCTCAGGCTTCCTTTGTGG
PRIMER_LEFT_3 314,20 PRIMER_RIGHT_3 544,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 203
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 0.4261
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CATGGCAAGCAAAACAGAGA PRIMER_RIGHT_4_SEQUENCE ATTGGCAGATTCGGATTTCA
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 1.00
PRIMER_LEFT_4_TM 59.988 PRIMER_RIGHT_4_TM 60.414
PRIMER_LEFT_4_PENALTY 0.012060 PRIMER_RIGHT_4_PENALTY 0.414033
PRIMER_LEFT_4 314,20 PRIMER_RIGHT_4 516,20
PRIMER_LEFT_4_END_STABILITY 6.4000 PRIMER_RIGHT_4_END_STABILITY 7.3000
PRIMER_LEFT_4_GC_PERCENT 45.000 PRIMER_RIGHT_4_GC_PERCENT 40.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00



insert: T-DNA_LB.SALK_022589.41.75.x

[Graphical View]
Sequence
ATATAGAACAAAAAGAATCATATCAAAAAGAGTGAAATACATTTATAAATTTTGAAGTAGAAAAATGGTGGATTCGTGAATAAACTGGCTTTCCTAAGAA
ACAGCATGGCTATTAACATACACTTTATTAAAAATCAGTTTTGATGTTTTAATTTTTCTATGACTATCTCTATCTTGTAAATAACATTATACAGTAATAA
GCATTGATGCAAATACGGTTTCGGGTTCGAGGGACTTTGTGTGTACATGATGACTACAGAGAGACAAGACCAAAAGTGTGGTCTATAGAAAAGAAGAACC
ATCCACGGGTTCAGCCAACTAAATAGGATCGAACCGGAGAGTCAGTGTATTGATCTTCAAGTTCCAATTCCATACAGTTTCTTGAATGGACTTGTCAAAG
ACCTGCAAGCTTATAAGATAGTTTCAATATCCGGTGAATGTGGCTGCGACAGACACTCGCATTATTAGCAAAATTCAGTGTTCTAAATATCACCTTAATT
AGTGTTGTTTGGGTTTTCTGTCCTGGTGCAATTAGCCAAAACACTAAAAGCAGAGACGAGGCCAGCCATGCCAACAACCTGTGTCCAGTTTCTTGTGCTA
AT
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 216
PRIMER_PAIR_PENALTY 0.3714
PRIMER_PAIR_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 55.000 PRIMER_RIGHT_GC_PERCENT 40.000
PRIMER_LEFT_POSITION 327 PRIMER_RIGHT_POSITION 542
PRIMER_LEFT_PENALTY 0.260757 PRIMER_RIGHT_PENALTY 0.110660
PRIMER_LEFT_SELF_END 3.00 PRIMER_RIGHT_SELF_END 2.00
PRIMER_LEFT_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_SEQUENCE TGTTTTGGCTAATTGCACCA
PRIMER_LEFT_END_STABILITY 6.7000 PRIMER_RIGHT_END_STABILITY 8.2000
PRIMER_LEFT_TM 60.261 PRIMER_RIGHT_TM 60.111
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 144
PRIMER_PAIR_PENALTY_1 0.6992
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 3.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.562 PRIMER_RIGHT_1_TM 60.261
PRIMER_LEFT_1_END_STABILITY 9.0000 PRIMER_RIGHT_1_END_STABILITY 8.3000
PRIMER_LEFT_1_GC_PERCENT 40.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_1_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 203,20 PRIMER_RIGHT_1 346,20
PRIMER_LEFT_1_SELF_END 3.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.438408 PRIMER_RIGHT_1_PENALTY 0.260757


Oligo 3
PRIMER_PRODUCT_SIZE_2 124
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.8059
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 6.4000 PRIMER_RIGHT_2_END_STABILITY 8.3000
PRIMER_LEFT_2_SEQUENCE GGTTCGAGGGACTTTGTGTG PRIMER_RIGHT_2_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 223,20 PRIMER_RIGHT_2 346,20
PRIMER_LEFT_2_TM 60.545 PRIMER_RIGHT_2_TM 60.261
PRIMER_LEFT_2_GC_PERCENT 55.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.545130 PRIMER_RIGHT_2_PENALTY 0.260757


Oligo 4
PRIMER_PRODUCT_SIZE_3 200
PRIMER_PAIR_PENALTY_3 0.8361
PRIMER_PAIR_3_COMPL_ANY 3.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.260757 PRIMER_RIGHT_3_PENALTY 0.575355
PRIMER_LEFT_3_SELF_END 3.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 8.8000
PRIMER_LEFT_3_GC_PERCENT 55.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 60.261 PRIMER_RIGHT_3_TM 59.425
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 3.00
PRIMER_LEFT_3_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_3_SEQUENCE ACCAGGACAGAAAACCCAAA
PRIMER_LEFT_3 327,20 PRIMER_RIGHT_3 526,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE_4 199
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 0.8361
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_4_SEQUENCE CCAGGACAGAAAACCCAAAC
PRIMER_LEFT_4_SELF_END 3.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 60.261 PRIMER_RIGHT_4_TM 59.425
PRIMER_LEFT_4_PENALTY 0.260757 PRIMER_RIGHT_4_PENALTY 0.575355
PRIMER_LEFT_4 327,20 PRIMER_RIGHT_4 525,20
PRIMER_LEFT_4_END_STABILITY 6.7000 PRIMER_RIGHT_4_END_STABILITY 7.0000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 50.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 3.00



insert: T-DNA_LB.SALK_022612.29.30.x

[Graphical View]
Sequence
GAAAAAAATTACACAACGAACGATCTGATAGCTTTTAGTATTCAAAAAAGCATATAGAACAAAAAGAATCATATCAAAAAGAGTGAAATACATTTATAAA
TTTTGAAGTAGAAAAATGGTGGATTCGTGAATAAACTGGCTTTCCTAAGAAACAGCATGGCTATTAACATACACTTTATTAAAAATCAGTTTTGATGTTT
TAATTTTTCTATGACTATCTCTATCTTGTAAATAACATTATACAGTAATAAGCATTGATGCAAATACGGTTTCGGGTTCGAGGGACTTTGTGTGTACATG
ATGACTACAGAGAGACAAGACCAAAAGTGTGGTCTATAGAAAAGAAGAACCATCCACGGGTTCAGCCAACTAAATAGGATCGAACCGGAGAGTCAGTGTA
TTGATCTTCAAGTTCCAATTCCATACAGTTTCTTGAATGGACTTGTCAAAGACCTGCAAGCTTATAAGATAGTTTCAATATCCGGTGAATGTGGCTGCGA
CAGACACTCGCATTATTAGCAAAATTCAGTGTTCTAAATATCACCTTAATTAGTGTTGTTTGGGTTTTCTGTCCTGGTGCAATTAGCCAAAACACTAAAA
GC
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 216
PRIMER_PAIR_PENALTY 0.3714
PRIMER_PAIR_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 55.000 PRIMER_RIGHT_GC_PERCENT 40.000
PRIMER_LEFT_POSITION 378 PRIMER_RIGHT_POSITION 593
PRIMER_LEFT_PENALTY 0.260757 PRIMER_RIGHT_PENALTY 0.110660
PRIMER_LEFT_SELF_END 3.00 PRIMER_RIGHT_SELF_END 2.00
PRIMER_LEFT_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_SEQUENCE TGTTTTGGCTAATTGCACCA
PRIMER_LEFT_END_STABILITY 6.7000 PRIMER_RIGHT_END_STABILITY 8.2000
PRIMER_LEFT_TM 60.261 PRIMER_RIGHT_TM 60.111
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 144
PRIMER_PAIR_PENALTY_1 0.6992
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 3.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.562 PRIMER_RIGHT_1_TM 60.261
PRIMER_LEFT_1_END_STABILITY 9.0000 PRIMER_RIGHT_1_END_STABILITY 8.3000
PRIMER_LEFT_1_GC_PERCENT 40.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_1_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 254,20 PRIMER_RIGHT_1 397,20
PRIMER_LEFT_1_SELF_END 3.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.438408 PRIMER_RIGHT_1_PENALTY 0.260757


Oligo 3
PRIMER_PRODUCT_SIZE_2 124
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.8059
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 6.4000 PRIMER_RIGHT_2_END_STABILITY 8.3000
PRIMER_LEFT_2_SEQUENCE GGTTCGAGGGACTTTGTGTG PRIMER_RIGHT_2_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 274,20 PRIMER_RIGHT_2 397,20
PRIMER_LEFT_2_TM 60.545 PRIMER_RIGHT_2_TM 60.261
PRIMER_LEFT_2_GC_PERCENT 55.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.545130 PRIMER_RIGHT_2_PENALTY 0.260757


Oligo 4
PRIMER_PRODUCT_SIZE_3 200
PRIMER_PAIR_PENALTY_3 0.8361
PRIMER_PAIR_3_COMPL_ANY 3.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.260757 PRIMER_RIGHT_3_PENALTY 0.575355
PRIMER_LEFT_3_SELF_END 3.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 8.8000
PRIMER_LEFT_3_GC_PERCENT 55.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 60.261 PRIMER_RIGHT_3_TM 59.425
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 3.00
PRIMER_LEFT_3_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_3_SEQUENCE ACCAGGACAGAAAACCCAAA
PRIMER_LEFT_3 378,20 PRIMER_RIGHT_3 577,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE_4 199
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 0.8361
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_4_SEQUENCE CCAGGACAGAAAACCCAAAC
PRIMER_LEFT_4_SELF_END 3.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 60.261 PRIMER_RIGHT_4_TM 59.425
PRIMER_LEFT_4_PENALTY 0.260757 PRIMER_RIGHT_4_PENALTY 0.575355
PRIMER_LEFT_4 378,20 PRIMER_RIGHT_4 576,20
PRIMER_LEFT_4_END_STABILITY 6.7000 PRIMER_RIGHT_4_END_STABILITY 7.0000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 50.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 3.00



insert: T-DNA_LB.GK-837G07-025717

[Graphical View]
Sequence
AAAATAATTTTTCGTCAAGAAAATTATATATTCATGATCTTTATATCTTACTTTCTATATTTTTTTATTAAATCAACGATATTTTTTATAGAAAAAAATT
ACACAACGAACGATCTGATAGCTTTTAGTATTCAAAAAAGCATATAGAACAAAAAGAATCATATCAAAAAGAGTGAAATACATTTATAAATTTTGAAGTA
GAAAAATGGTGGATTCGTGAATAAACTGGCTTTCCTAAGAAACAGCATGGCTATTAACATACACTTTATTAAAAATCAGTTTTGATGTTTTAATTTTTCT
ATGACTATCTCTATCTTGTAAATAACATTATACAGTAATAAGCATTGATGCAAATACGGTTTCGGGTTCGAGGGACTTTGTGTGTACATGATGACTACAG
AGAGACAAGACCAAAAGTGTGGTCTATAGAAAAGAAGAACCATCCACGGGTTCAGCCAACTAAATAGGATCGAACCGGAGAGTCAGTGTATTGATCTTCA
AGTTCCAATTCCATACAGTTTCTTGAATGGACTTGTCAAAGACCTGCAAGCTTATAAGATAGTTTCAATATCCGGTGAATGTGGCTGCGACAGACACTCG
CA
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 144
PRIMER_PAIR_PENALTY 0.6992
PRIMER_PAIR_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 40.000 PRIMER_RIGHT_GC_PERCENT 55.000
PRIMER_LEFT_POSITION 344 PRIMER_RIGHT_POSITION 487
PRIMER_LEFT_PENALTY 0.438408 PRIMER_RIGHT_PENALTY 0.260757
PRIMER_LEFT_SELF_END 3.00 PRIMER_RIGHT_SELF_END 2.00
PRIMER_LEFT_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_END_STABILITY 9.0000 PRIMER_RIGHT_END_STABILITY 8.3000
PRIMER_LEFT_TM 59.562 PRIMER_RIGHT_TM 60.261
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 124
PRIMER_PAIR_PENALTY_1 0.8059
PRIMER_PAIR_1_COMPL_END 0.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.545 PRIMER_RIGHT_1_TM 60.261
PRIMER_LEFT_1_END_STABILITY 6.4000 PRIMER_RIGHT_1_END_STABILITY 8.3000
PRIMER_LEFT_1_GC_PERCENT 55.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE GGTTCGAGGGACTTTGTGTG PRIMER_RIGHT_1_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 364,20 PRIMER_RIGHT_1 487,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.545130 PRIMER_RIGHT_1_PENALTY 0.260757


Oligo 3
PRIMER_PRODUCT_SIZE_2 118
PRIMER_PAIR_2_COMPL_ANY 5.00
PRIMER_PAIR_2_COMPL_END 1.00
PRIMER_PAIR_PENALTY_2 0.9881
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 6.7000 PRIMER_RIGHT_2_END_STABILITY 5.8000
PRIMER_LEFT_2_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_2_SEQUENCE AGCCACATTCACCGGATATT
PRIMER_LEFT_2_SELF_END 3.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 468,20 PRIMER_RIGHT_2 585,20
PRIMER_LEFT_2_TM 60.261 PRIMER_RIGHT_2_TM 59.273
PRIMER_LEFT_2_GC_PERCENT 55.000 PRIMER_RIGHT_2_GC_PERCENT 45.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.260757 PRIMER_RIGHT_2_PENALTY 0.727304


Oligo 4
PRIMER_PRODUCT_SIZE_3 116
PRIMER_PAIR_PENALTY_3 1.0603
PRIMER_PAIR_3_COMPL_ANY 5.00
PRIMER_PAIR_3_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.260757 PRIMER_RIGHT_3_PENALTY 0.799535
PRIMER_LEFT_3_SELF_END 3.00 PRIMER_RIGHT_3_SELF_END 2.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 6.9000
PRIMER_LEFT_3_GC_PERCENT 55.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 60.261 PRIMER_RIGHT_3_TM 59.200
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE ATCGAACCGGAGAGTCAGTG PRIMER_RIGHT_3_SEQUENCE CCACATTCACCGGATATTGA
PRIMER_LEFT_3 468,20 PRIMER_RIGHT_3 583,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE_4 242
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 1.1657
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_4_SEQUENCE AGCCACATTCACCGGATATT
PRIMER_LEFT_4_SELF_END 3.00 PRIMER_RIGHT_4_SELF_END 2.00
PRIMER_LEFT_4_TM 59.562 PRIMER_RIGHT_4_TM 59.273
PRIMER_LEFT_4_PENALTY 0.438408 PRIMER_RIGHT_4_PENALTY 0.727304
PRIMER_LEFT_4 344,20 PRIMER_RIGHT_4 585,20
PRIMER_LEFT_4_END_STABILITY 9.0000 PRIMER_RIGHT_4_END_STABILITY 5.8000
PRIMER_LEFT_4_GC_PERCENT 40.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00



insert: T-DNA_LB.SALK_095363

[Graphical View]
Sequence
ATTTTTTTTCCTCAGAAGCCCAGATGAGAAAATTACGTTTTTGGACACGTATTGCTCTCTCAATATAGATTTTCTGAATATCAAGAAAATAATTTTTCGT
CAAGAAAATTATATATTCATGATCTTTATATCTTACTTTCTATATTTTTTTATTAAATCAACGATATTTTTTATAGAAAAAAATTACACAACGAACGATC
TGATAGCTTTTAGTATTCAAAAAAGCATATAGAACAAAAAGAATCATATCAAAAAGAGTGAAATACATTTATAAATTTTGAAGTAGAAAAATGGTGGATT
CGTGAATAAACTGGCTTTCCTAAGAAACAGCATGGCTATTAACATACACTTTATTAAAAATCAGTTTTGATGTTTTAATTTTTCTATGACTATCTCTATC
TTGTAAATAACATTATACAGTAATAAGCATTGATGCAAATACGGTTTCGGGTTCGAGGGACTTTGTGTGTACATGATGACTACAGAGAGACAAGACCAAA
AGTGTGGTCTATAGAAAAGAAGAACCATCCACGGGTTCAGCCAACTAAATAGGATCGAACCGGAGAGTCAGTGTATTGATCTTCAAGTTCCAATTCCATA
CA
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 144
PRIMER_PAIR_PENALTY 0.6992
PRIMER_PAIR_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 40.000 PRIMER_RIGHT_GC_PERCENT 55.000
PRIMER_LEFT_POSITION 429 PRIMER_RIGHT_POSITION 572
PRIMER_LEFT_PENALTY 0.438408 PRIMER_RIGHT_PENALTY 0.260757
PRIMER_LEFT_SELF_END 3.00 PRIMER_RIGHT_SELF_END 2.00
PRIMER_LEFT_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_END_STABILITY 9.0000 PRIMER_RIGHT_END_STABILITY 8.3000
PRIMER_LEFT_TM 59.562 PRIMER_RIGHT_TM 60.261
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 124
PRIMER_PAIR_PENALTY_1 0.8059
PRIMER_PAIR_1_COMPL_END 0.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.545 PRIMER_RIGHT_1_TM 60.261
PRIMER_LEFT_1_END_STABILITY 6.4000 PRIMER_RIGHT_1_END_STABILITY 8.3000
PRIMER_LEFT_1_GC_PERCENT 55.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE GGTTCGAGGGACTTTGTGTG PRIMER_RIGHT_1_SEQUENCE CACTGACTCTCCGGTTCGAT
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 449,20 PRIMER_RIGHT_1 572,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.545130 PRIMER_RIGHT_1_PENALTY 0.260757


Oligo 3
PRIMER_PRODUCT_SIZE_2 107
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 1.1840
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 9.0000 PRIMER_RIGHT_2_END_STABILITY 7.0000
PRIMER_LEFT_2_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_2_SEQUENCE ACCCGTGGATGGTTCTTCTT
PRIMER_LEFT_2_SELF_END 3.00 PRIMER_RIGHT_2_SELF_END 0.00
PRIMER_LEFT_2 429,20 PRIMER_RIGHT_2 535,20
PRIMER_LEFT_2_TM 59.562 PRIMER_RIGHT_2_TM 60.746
PRIMER_LEFT_2_GC_PERCENT 40.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 3.00
PRIMER_LEFT_2_PENALTY 0.438408 PRIMER_RIGHT_2_PENALTY 0.745579


Oligo 4
PRIMER_PRODUCT_SIZE_3 108
PRIMER_PAIR_PENALTY_3 1.1840
PRIMER_PAIR_3_COMPL_ANY 5.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.438408 PRIMER_RIGHT_3_PENALTY 0.745579
PRIMER_LEFT_3_SELF_END 3.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 9.0000 PRIMER_RIGHT_3_END_STABILITY 6.7000
PRIMER_LEFT_3_GC_PERCENT 40.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 59.562 PRIMER_RIGHT_3_TM 60.746
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_3_SEQUENCE AACCCGTGGATGGTTCTTCT
PRIMER_LEFT_3 429,20 PRIMER_RIGHT_3 536,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE_4 140
PRIMER_PAIR_4_COMPL_END 2.00
PRIMER_PAIR_PENALTY_4 1.1999
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE TTGATGCAAATACGGTTTCG PRIMER_RIGHT_4_SEQUENCE GACTCTCCGGTTCGATCCTA
PRIMER_LEFT_4_SELF_END 3.00 PRIMER_RIGHT_4_SELF_END 2.00
PRIMER_LEFT_4_TM 59.562 PRIMER_RIGHT_4_TM 59.239
PRIMER_LEFT_4_PENALTY 0.438408 PRIMER_RIGHT_4_PENALTY 0.761472
PRIMER_LEFT_4 429,20 PRIMER_RIGHT_4 568,20
PRIMER_LEFT_4_END_STABILITY 9.0000 PRIMER_RIGHT_4_END_STABILITY 7.2000
PRIMER_LEFT_4_GC_PERCENT 40.000 PRIMER_RIGHT_4_GC_PERCENT 55.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 6.00