gene: At3g17970

insert: T-DNA_LB.SALK_146190.18.00.x

[Graphical View]
Sequence
AATGTTAAAGCCTATTTACGAAGAGGAACCGCAAGAGAAATGTTAGGCGATTGCAAGGGAGCCATAGAAGGTAAGCTTTAAAGAACATAATCTGATTGTT
TTTTCGCCTTGTAGAATCTGGCAGAATATAACATAAACTCTGCAGATTTCAGATACGCACTTGTGCTGGAACCAAACAACAAACGAGCGTCTCTCTCAGC
AGAGAGACTGAGAAAATTCCAGTGAGCTTCTTTTGTCCGTACCTCACCGATCATTGAGAAAACGTTTGTTCCTTAGAATTTGTCCAAGAGTATAGGCCAA
TTTTGTCAACACTTGTCTTTTGCGTCTGATATTTAGGCATTTTATCTAGAGATACCTTTTGCAAGTCCGCCGAGAATGGAAAATTATAATATCCGATGTT
TATTTTAAACGTTCTCTAAAATTGGATATCAAAAAGTAATGTTAATCTTAAACACCGGATACCGGCATTCCCGGTCGACTTCTAGTCAGCGTAGCGTCAA
AAATAAGGTCATGTCTTTAACTATCTTGTCTTTATCATTTTCTTTTCTCGCACGTTATCTCCTCCCCACGACTCAAAAAAAATAAACAGAAAAAAAGAGG
AG
Oligo 1
PRIMER_PAIR_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE 299
PRIMER_PAIR_PENALTY 0.2025
PRIMER_PAIR_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 40.000 PRIMER_RIGHT_GC_PERCENT 50.000
PRIMER_LEFT_POSITION 166 PRIMER_RIGHT_POSITION 464
PRIMER_LEFT_PENALTY 0.019325 PRIMER_RIGHT_PENALTY 0.183145
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 3.00
PRIMER_LEFT_SEQUENCE TGGAACCAAACAACAAACGA PRIMER_RIGHT_SEQUENCE CCGGTATCCGGTGTTTAAGA
PRIMER_LEFT_END_STABILITY 8.4000 PRIMER_RIGHT_END_STABILITY 6.0000
PRIMER_LEFT_TM 59.981 PRIMER_RIGHT_TM 59.817
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 6.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 145
PRIMER_PAIR_PENALTY_1 0.2222
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 3.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.096 PRIMER_RIGHT_1_TM 60.127
PRIMER_LEFT_1_END_STABILITY 8.5000 PRIMER_RIGHT_1_END_STABILITY 8.5000
PRIMER_LEFT_1_GC_PERCENT 45.000 PRIMER_RIGHT_1_GC_PERCENT 40.000
PRIMER_LEFT_1_SEQUENCE AATGTTAGGCGATTGCAAGG PRIMER_RIGHT_1_SEQUENCE TTTGTTGTTTGGTTCCAGCA
PRIMER_LEFT_1_SELF_ANY 6.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 38,20 PRIMER_RIGHT_1 182,20
PRIMER_LEFT_1_SELF_END 2.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.095629 PRIMER_RIGHT_1_PENALTY 0.126538


Oligo 3
PRIMER_PRODUCT_SIZE_2 133
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 1.00
PRIMER_PAIR_PENALTY_2 0.2247
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 8.5000 PRIMER_RIGHT_2_END_STABILITY 5.3000
PRIMER_LEFT_2_SEQUENCE AATGTTAGGCGATTGCAAGG PRIMER_RIGHT_2_SEQUENCE TTCCAGCACAAGTGCGTATC
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 0.00
PRIMER_LEFT_2 38,20 PRIMER_RIGHT_2 170,20
PRIMER_LEFT_2_TM 60.096 PRIMER_RIGHT_2_TM 59.871
PRIMER_LEFT_2_GC_PERCENT 45.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 6.00 PRIMER_RIGHT_2_SELF_ANY 6.00
PRIMER_LEFT_2_PENALTY 0.095629 PRIMER_RIGHT_2_PENALTY 0.129070


Oligo 4
PRIMER_PRODUCT_SIZE_3 155
PRIMER_PAIR_PENALTY_3 0.2814
PRIMER_PAIR_3_COMPL_ANY 3.00
PRIMER_PAIR_3_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.154878 PRIMER_RIGHT_3_PENALTY 0.126538
PRIMER_LEFT_3_SELF_END 2.00 PRIMER_RIGHT_3_SELF_END 2.00
PRIMER_LEFT_3_END_STABILITY 8.7000 PRIMER_RIGHT_3_END_STABILITY 8.5000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 40.000
PRIMER_LEFT_3_TM 59.845 PRIMER_RIGHT_3_TM 60.127
PRIMER_LEFT_3_SELF_ANY 2.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE CCGCAAGAGAAATGTTAGGC PRIMER_RIGHT_3_SEQUENCE TTTGTTGTTTGGTTCCAGCA
PRIMER_LEFT_3 28,20 PRIMER_RIGHT_3 182,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 143
PRIMER_PAIR_4_COMPL_END 3.00
PRIMER_PAIR_PENALTY_4 0.2839
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CCGCAAGAGAAATGTTAGGC PRIMER_RIGHT_4_SEQUENCE TTCCAGCACAAGTGCGTATC
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 59.845 PRIMER_RIGHT_4_TM 59.871
PRIMER_LEFT_4_PENALTY 0.154878 PRIMER_RIGHT_4_PENALTY 0.129070
PRIMER_LEFT_4 28,20 PRIMER_RIGHT_4 170,20
PRIMER_LEFT_4_END_STABILITY 8.7000 PRIMER_RIGHT_4_END_STABILITY 5.3000
PRIMER_LEFT_4_GC_PERCENT 50.000 PRIMER_RIGHT_4_GC_PERCENT 50.000
PRIMER_LEFT_4_SELF_ANY 2.00 PRIMER_RIGHT_4_SELF_ANY 6.00



insert: T-DNA_LB.GK-764C01-024030

[Graphical View]
Sequence
ATGACATGTCCACAACTTGAGCGAGTTAGTTTATATGTCTCTTGACTAATCTCTAATGGTTCCCAACTCTGTAACGTTATGTATCAAGGATGATGGCATT
CTGGTAATTCCAACATTGCCAGCTGTTCCTCCAAAACTTGGTAGCAAGGAGATAACATCTGAAGACTATCAAAACCGAGCTTCAAGTCTGCTTAGCATTG
CTAGCATATCAGGATGTTGTCAGGTTAAATCTCTCTCTTTCTCTCTCTCACCTGCCATGCTAGTAAAGTTCCCCTCACACATGTTAAAAGCAACCACTGA
CCAGTCAGTGTTAGCAAAACCAATCCACAGTATATGTGTCTTACATTTATCGTGCTGATGATTCAGGTGACTGTGCCACTCGGACATCATGAGAAATGCC
CTATTTCAGTTTCTTTCATAGGAAGACACGGTGGTGACCGCTTTTTACTAGATACAGTGCAGACAATGTATCCATCTTTGCAAGAGTACTCGAGTATTGT
TACCGATCCCAAATCTTCTAAAAAGGCTATCACCAAAGAAGAGTCAGCCGAGATTGCCAAAGAGAAGGTCAATTAATATCCATTGCATGTCTCATTTTCT
TA
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 170
PRIMER_PAIR_PENALTY 0.3000
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 50.000
PRIMER_LEFT_POSITION 381 PRIMER_RIGHT_POSITION 550
PRIMER_LEFT_PENALTY 0.289438 PRIMER_RIGHT_PENALTY 0.010545
PRIMER_LEFT_SELF_END 1.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE GGACATCATGAGAAATGCCC PRIMER_RIGHT_SEQUENCE TCGGCTGACTCTTCTTTGGT
PRIMER_LEFT_END_STABILITY 11.2000 PRIMER_RIGHT_END_STABILITY 8.2000
PRIMER_LEFT_TM 60.289 PRIMER_RIGHT_TM 59.989
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 6.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 186
PRIMER_PAIR_PENALTY_1 0.3411
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 5.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.289 PRIMER_RIGHT_1_TM 59.948
PRIMER_LEFT_1_END_STABILITY 11.2000 PRIMER_RIGHT_1_END_STABILITY 9.9000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE GGACATCATGAGAAATGCCC PRIMER_RIGHT_1_SEQUENCE CTTCTCTTTGGCAATCTCGG
PRIMER_LEFT_1_SELF_ANY 6.00 PRIMER_RIGHT_1_SELF_ANY 5.00
PRIMER_LEFT_1 381,20 PRIMER_RIGHT_1 566,20
PRIMER_LEFT_1_SELF_END 1.00 PRIMER_RIGHT_1_SELF_END 1.00
PRIMER_LEFT_1_PENALTY 0.289438 PRIMER_RIGHT_1_PENALTY 0.051674


Oligo 3
PRIMER_PRODUCT_SIZE_2 187
PRIMER_PAIR_2_COMPL_ANY 5.00
PRIMER_PAIR_2_COMPL_END 3.00
PRIMER_PAIR_PENALTY_2 0.3411
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 11.2000 PRIMER_RIGHT_2_END_STABILITY 8.4000
PRIMER_LEFT_2_SEQUENCE GGACATCATGAGAAATGCCC PRIMER_RIGHT_2_SEQUENCE CCTTCTCTTTGGCAATCTCG
PRIMER_LEFT_2_SELF_END 1.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 381,20 PRIMER_RIGHT_2 567,20
PRIMER_LEFT_2_TM 60.289 PRIMER_RIGHT_2_TM 59.948
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 6.00 PRIMER_RIGHT_2_SELF_ANY 5.00
PRIMER_LEFT_2_PENALTY 0.289438 PRIMER_RIGHT_2_PENALTY 0.051674


Oligo 4
PRIMER_PRODUCT_SIZE_3 176
PRIMER_PAIR_PENALTY_3 0.3798
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 3.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.369208 PRIMER_RIGHT_3_PENALTY 0.010545
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 8.2000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 59.631 PRIMER_RIGHT_3_TM 59.989
PRIMER_LEFT_3_SELF_ANY 6.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE CCACTCGGACATCATGAGAA PRIMER_RIGHT_3_SEQUENCE TCGGCTGACTCTTCTTTGGT
PRIMER_LEFT_3 375,20 PRIMER_RIGHT_3 550,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE_4 270
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 0.3913
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE AACATTGCCAGCTGTTCCTC PRIMER_RIGHT_4_SEQUENCE GAGTGGCACAGTCACCTGAA
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 2.00
PRIMER_LEFT_4_TM 60.263 PRIMER_RIGHT_4_TM 59.872
PRIMER_LEFT_4_PENALTY 0.262854 PRIMER_RIGHT_4_PENALTY 0.128480
PRIMER_LEFT_4 111,20 PRIMER_RIGHT_4 380,20
PRIMER_LEFT_4_END_STABILITY 7.9000 PRIMER_RIGHT_4_END_STABILITY 7.0000
PRIMER_LEFT_4_GC_PERCENT 50.000 PRIMER_RIGHT_4_GC_PERCENT 55.000
PRIMER_LEFT_4_SELF_ANY 6.00 PRIMER_RIGHT_4_SELF_ANY 5.00



insert: T-DNA_LB.GK-763C01-023908

[Graphical View]
Sequence
TTCGCTAGGACAAAAGCCATTGCTAACACGAAGGTCTCAACATCGAGACTACTGGCGAATGTGATGCAGCTCCTTCAAAGGTGTGCCTATTGTTTATTCA
CCTACAGAGGATGAAAATATCTTAGTTTTGAACACTCTATAACAATATTGGCATTGTAGGCACGAGTTCCTTCAAAATCATGGGGATTGGATCAACACAG
TGAAGCCAGCCATTGATCCTGTGATTTTATCACAAGTGTGTGAGAACCCAGAACTAACCAATGAAGAGACTGAGAACCTGAATGCTATCAGAAACGAGAC
TCGAGTGGCTATTGGTTCACTTCTCAAGGTATTCCTGCTCTTCCATGACATGTCCACAACTTGAGCGAGTTAGTTTATATGTCTCTTGACTAATCTCTAA
TGGTTCCCAACTCTGTAACGTTATGTATCAAGGATGATGGCATTCTGGTAATTCCAACATTGCCAGCTGTTCCTCCAAAACTTGGTAGCAAGGAGATAAC
ATCTGAAGACTATCAAAACCGAGCTTCAAGTCTGCTTAGCATTGCTAGCATATCAGGATGTTGTCAGGTTAAATCTCTCTCTTTCTCTCTCTCACCTGCC
AT
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 179
PRIMER_PAIR_PENALTY 0.1876
PRIMER_PAIR_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 40.000
PRIMER_LEFT_POSITION 15 PRIMER_RIGHT_POSITION 193
PRIMER_LEFT_PENALTY 0.139753 PRIMER_RIGHT_PENALTY 0.047806
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 1.00
PRIMER_LEFT_SEQUENCE GCCATTGCTAACACGAAGGT PRIMER_RIGHT_SEQUENCE TGATCCAATCCCCATGATTT
PRIMER_LEFT_END_STABILITY 7.9000 PRIMER_RIGHT_END_STABILITY 6.9000
PRIMER_LEFT_TM 60.140 PRIMER_RIGHT_TM 59.952
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 180
PRIMER_PAIR_PENALTY_1 0.1876
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.140 PRIMER_RIGHT_1_TM 59.952
PRIMER_LEFT_1_END_STABILITY 7.9000 PRIMER_RIGHT_1_END_STABILITY 6.9000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 40.000
PRIMER_LEFT_1_SEQUENCE GCCATTGCTAACACGAAGGT PRIMER_RIGHT_1_SEQUENCE TTGATCCAATCCCCATGATT
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 15,20 PRIMER_RIGHT_1 194,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 3.00
PRIMER_LEFT_1_PENALTY 0.139753 PRIMER_RIGHT_1_PENALTY 0.047806


Oligo 3
PRIMER_PRODUCT_SIZE_2 193
PRIMER_PAIR_2_COMPL_ANY 3.00
PRIMER_PAIR_2_COMPL_END 2.00
PRIMER_PAIR_PENALTY_2 0.2027
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 8.4000 PRIMER_RIGHT_2_END_STABILITY 6.9000
PRIMER_LEFT_2_SEQUENCE TCGCTAGGACAAAAGCCATT PRIMER_RIGHT_2_SEQUENCE TGATCCAATCCCCATGATTT
PRIMER_LEFT_2_SELF_END 3.00 PRIMER_RIGHT_2_SELF_END 1.00
PRIMER_LEFT_2 1,20 PRIMER_RIGHT_2 193,20
PRIMER_LEFT_2_TM 59.845 PRIMER_RIGHT_2_TM 59.952
PRIMER_LEFT_2_GC_PERCENT 45.000 PRIMER_RIGHT_2_GC_PERCENT 40.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 4.00
PRIMER_LEFT_2_PENALTY 0.154878 PRIMER_RIGHT_2_PENALTY 0.047806


Oligo 4
PRIMER_PRODUCT_SIZE_3 194
PRIMER_PAIR_PENALTY_3 0.2027
PRIMER_PAIR_3_COMPL_ANY 3.00
PRIMER_PAIR_3_COMPL_END 3.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.154878 PRIMER_RIGHT_3_PENALTY 0.047806
PRIMER_LEFT_3_SELF_END 2.00 PRIMER_RIGHT_3_SELF_END 1.00
PRIMER_LEFT_3_END_STABILITY 9.6000 PRIMER_RIGHT_3_END_STABILITY 6.9000
PRIMER_LEFT_3_GC_PERCENT 45.000 PRIMER_RIGHT_3_GC_PERCENT 40.000
PRIMER_LEFT_3_TM 59.845 PRIMER_RIGHT_3_TM 59.952
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE TTCGCTAGGACAAAAGCCAT PRIMER_RIGHT_3_SEQUENCE TGATCCAATCCCCATGATTT
PRIMER_LEFT_3 0,20 PRIMER_RIGHT_3 193,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 194
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 0.2027
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE TCGCTAGGACAAAAGCCATT PRIMER_RIGHT_4_SEQUENCE TTGATCCAATCCCCATGATT
PRIMER_LEFT_4_SELF_END 3.00 PRIMER_RIGHT_4_SELF_END 3.00
PRIMER_LEFT_4_TM 59.845 PRIMER_RIGHT_4_TM 59.952
PRIMER_LEFT_4_PENALTY 0.154878 PRIMER_RIGHT_4_PENALTY 0.047806
PRIMER_LEFT_4 1,20 PRIMER_RIGHT_4 194,20
PRIMER_LEFT_4_END_STABILITY 8.4000 PRIMER_RIGHT_4_END_STABILITY 6.9000
PRIMER_LEFT_4_GC_PERCENT 45.000 PRIMER_RIGHT_4_GC_PERCENT 40.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00



insert: T-DNA_LB.GK-624B07-022319

[Graphical View]
Sequence
AATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAACCCTCGAGTGATGTTAAAAAAAGGAAGAAGGAAAAAGAATAAA
AACTCAAAGGGAACGCAACACACTAAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTAAGCAGCAGCAGCAGCAGCTAGAAC
AAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGCTGAAGAAGACC
GTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCACCGGTCTAACCT
TTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCTGAATGATTTTT
TCCTTGGGTAGAGTCAAAATTTTGTTTGACAAATGTTGGAATCTGCGTTGACTCGACTCGTAATCTTCTCCGGCGCACTACATGTCTGATTCTGAGCTTT
CG
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 163
PRIMER_PAIR_PENALTY 0.1424
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 331 PRIMER_RIGHT_POSITION 493
PRIMER_LEFT_PENALTY 0.073866 PRIMER_RIGHT_PENALTY 0.068521
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_END_STABILITY 10.9000 PRIMER_RIGHT_END_STABILITY 8.8000
PRIMER_LEFT_TM 60.074 PRIMER_RIGHT_TM 59.931
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 6.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 110
PRIMER_PAIR_PENALTY_1 0.1622
PRIMER_PAIR_1_COMPL_END 3.00
PRIMER_PAIR_1_COMPL_ANY 6.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.074 PRIMER_RIGHT_1_TM 60.088
PRIMER_LEFT_1_END_STABILITY 10.9000 PRIMER_RIGHT_1_END_STABILITY 7.9000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_1_SEQUENCE GGGAAACAAGTCAACGGAGA
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 2.00
PRIMER_LEFT_1 331,20 PRIMER_RIGHT_1 440,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.073866 PRIMER_RIGHT_1_PENALTY 0.088296


Oligo 3
PRIMER_PRODUCT_SIZE_2 100
PRIMER_PAIR_2_COMPL_ANY 3.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.2023
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 10.9000 PRIMER_RIGHT_2_END_STABILITY 7.8000
PRIMER_LEFT_2_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_2_SEQUENCE TCAACGGAGACTCACACGTC
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 3.00
PRIMER_LEFT_2 331,20 PRIMER_RIGHT_2 430,20
PRIMER_LEFT_2_TM 60.074 PRIMER_RIGHT_2_TM 59.872
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 5.00
PRIMER_LEFT_2_PENALTY 0.073866 PRIMER_RIGHT_2_PENALTY 0.128480


Oligo 4
PRIMER_PRODUCT_SIZE_3 203
PRIMER_PAIR_PENALTY_3 0.2246
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.156061 PRIMER_RIGHT_3_PENALTY 0.068521
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 8.8000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 59.844 PRIMER_RIGHT_3_TM 59.931
PRIMER_LEFT_3_SELF_ANY 5.00 PRIMER_RIGHT_3_SELF_ANY 6.00
PRIMER_LEFT_3_SEQUENCE AAGAAGACCGTCCGTGAAGA PRIMER_RIGHT_3_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_3 291,20 PRIMER_RIGHT_3 493,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 204
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.2246
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE GAAGAAGACCGTCCGTGAAG PRIMER_RIGHT_4_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 59.844 PRIMER_RIGHT_4_TM 59.931
PRIMER_LEFT_4_PENALTY 0.156061 PRIMER_RIGHT_4_PENALTY 0.068521
PRIMER_LEFT_4 290,20 PRIMER_RIGHT_4 493,20
PRIMER_LEFT_4_END_STABILITY 7.0000 PRIMER_RIGHT_4_END_STABILITY 8.8000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 5.00 PRIMER_RIGHT_4_SELF_ANY 6.00



insert: T-DNA_LB.GK-624B07-022001

[Graphical View]
Sequence
TTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAACCCTCGAGTGATGTTAAAAAAAGGAAGAAGGA
AAAAGAATAAAAACTCAAAGGGAACGCAACACACTAAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTAAGCAGCAGCAGCA
GCAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGC
TGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCAC
CGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCT
GAATGATTTTTTCCTTGGGTAGAGTCAAAATTTTGTTTGACAAATGTTGGAATCTGCGTTGACTCGACTCGTAATCTTCTCCGGCGCACTACATGTCTGA
TT
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 163
PRIMER_PAIR_PENALTY 0.1424
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 342 PRIMER_RIGHT_POSITION 504
PRIMER_LEFT_PENALTY 0.073866 PRIMER_RIGHT_PENALTY 0.068521
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_END_STABILITY 10.9000 PRIMER_RIGHT_END_STABILITY 8.8000
PRIMER_LEFT_TM 60.074 PRIMER_RIGHT_TM 59.931
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 6.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 110
PRIMER_PAIR_PENALTY_1 0.1622
PRIMER_PAIR_1_COMPL_END 3.00
PRIMER_PAIR_1_COMPL_ANY 6.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.074 PRIMER_RIGHT_1_TM 60.088
PRIMER_LEFT_1_END_STABILITY 10.9000 PRIMER_RIGHT_1_END_STABILITY 7.9000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_1_SEQUENCE GGGAAACAAGTCAACGGAGA
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 2.00
PRIMER_LEFT_1 342,20 PRIMER_RIGHT_1 451,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.073866 PRIMER_RIGHT_1_PENALTY 0.088296


Oligo 3
PRIMER_PRODUCT_SIZE_2 100
PRIMER_PAIR_2_COMPL_ANY 3.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.2023
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 10.9000 PRIMER_RIGHT_2_END_STABILITY 7.8000
PRIMER_LEFT_2_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_2_SEQUENCE TCAACGGAGACTCACACGTC
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 3.00
PRIMER_LEFT_2 342,20 PRIMER_RIGHT_2 441,20
PRIMER_LEFT_2_TM 60.074 PRIMER_RIGHT_2_TM 59.872
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 5.00
PRIMER_LEFT_2_PENALTY 0.073866 PRIMER_RIGHT_2_PENALTY 0.128480


Oligo 4
PRIMER_PRODUCT_SIZE_3 203
PRIMER_PAIR_PENALTY_3 0.2246
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.156061 PRIMER_RIGHT_3_PENALTY 0.068521
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 8.8000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 59.844 PRIMER_RIGHT_3_TM 59.931
PRIMER_LEFT_3_SELF_ANY 5.00 PRIMER_RIGHT_3_SELF_ANY 6.00
PRIMER_LEFT_3_SEQUENCE AAGAAGACCGTCCGTGAAGA PRIMER_RIGHT_3_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_3 302,20 PRIMER_RIGHT_3 504,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 204
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.2246
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE GAAGAAGACCGTCCGTGAAG PRIMER_RIGHT_4_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 59.844 PRIMER_RIGHT_4_TM 59.931
PRIMER_LEFT_4_PENALTY 0.156061 PRIMER_RIGHT_4_PENALTY 0.068521
PRIMER_LEFT_4 301,20 PRIMER_RIGHT_4 504,20
PRIMER_LEFT_4_END_STABILITY 7.0000 PRIMER_RIGHT_4_END_STABILITY 8.8000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 5.00 PRIMER_RIGHT_4_SELF_ANY 6.00



insert: T-DNA_LB.GK-624B07-022315

[Graphical View]
Sequence
TTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAACCCTCGAGTGATGTTAAAAAAAGGAAGAAGGA
AAAAGAATAAAAACTCAAAGGGAACGCAACACACTAAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTAAGCAGCAGCAGCA
GCAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGC
TGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCAC
CGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCT
GAATGATTTTTTCCTTGGGTAGAGTCAAAATTTTGTTTGACAAATGTTGGAATCTGCGTTGACTCGACTCGTAATCTTCTCCGGCGCACTACATGTCTGA
TT
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 163
PRIMER_PAIR_PENALTY 0.1424
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 342 PRIMER_RIGHT_POSITION 504
PRIMER_LEFT_PENALTY 0.073866 PRIMER_RIGHT_PENALTY 0.068521
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_END_STABILITY 10.9000 PRIMER_RIGHT_END_STABILITY 8.8000
PRIMER_LEFT_TM 60.074 PRIMER_RIGHT_TM 59.931
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 6.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 110
PRIMER_PAIR_PENALTY_1 0.1622
PRIMER_PAIR_1_COMPL_END 3.00
PRIMER_PAIR_1_COMPL_ANY 6.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.074 PRIMER_RIGHT_1_TM 60.088
PRIMER_LEFT_1_END_STABILITY 10.9000 PRIMER_RIGHT_1_END_STABILITY 7.9000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_1_SEQUENCE GGGAAACAAGTCAACGGAGA
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 2.00
PRIMER_LEFT_1 342,20 PRIMER_RIGHT_1 451,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.073866 PRIMER_RIGHT_1_PENALTY 0.088296


Oligo 3
PRIMER_PRODUCT_SIZE_2 100
PRIMER_PAIR_2_COMPL_ANY 3.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.2023
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 10.9000 PRIMER_RIGHT_2_END_STABILITY 7.8000
PRIMER_LEFT_2_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_2_SEQUENCE TCAACGGAGACTCACACGTC
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 3.00
PRIMER_LEFT_2 342,20 PRIMER_RIGHT_2 441,20
PRIMER_LEFT_2_TM 60.074 PRIMER_RIGHT_2_TM 59.872
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 5.00
PRIMER_LEFT_2_PENALTY 0.073866 PRIMER_RIGHT_2_PENALTY 0.128480


Oligo 4
PRIMER_PRODUCT_SIZE_3 203
PRIMER_PAIR_PENALTY_3 0.2246
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.156061 PRIMER_RIGHT_3_PENALTY 0.068521
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 8.8000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 59.844 PRIMER_RIGHT_3_TM 59.931
PRIMER_LEFT_3_SELF_ANY 5.00 PRIMER_RIGHT_3_SELF_ANY 6.00
PRIMER_LEFT_3_SEQUENCE AAGAAGACCGTCCGTGAAGA PRIMER_RIGHT_3_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_3 302,20 PRIMER_RIGHT_3 504,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 204
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.2246
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE GAAGAAGACCGTCCGTGAAG PRIMER_RIGHT_4_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 59.844 PRIMER_RIGHT_4_TM 59.931
PRIMER_LEFT_4_PENALTY 0.156061 PRIMER_RIGHT_4_PENALTY 0.068521
PRIMER_LEFT_4 301,20 PRIMER_RIGHT_4 504,20
PRIMER_LEFT_4_END_STABILITY 7.0000 PRIMER_RIGHT_4_END_STABILITY 8.8000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 5.00 PRIMER_RIGHT_4_SELF_ANY 6.00



insert: T-DNA_LB.GK-646G11-023221

[Graphical View]
Sequence
TTAATAAAATTAATTTTTTGTTTTACAACGTGAATAAAAAACTGTTGACAAAACGAAAATAAAATAAACCCTCGAGTGATGTTAAAAAAAGGAAGAAGGA
AAAAGAATAAAAACTCAAAGGGAACGCAACACACTAAACAAAAACTCTTTGGCGTCCTTCTCCTTAGGGTCTATCTCTCTTATCTTAAGCAGCAGCAGCA
GCAGCTAGAACAAAAACGTCACCGATGGCGTCTCAAGCTGCGAATCTCTGGGTTCTTCTAGGTTTAGGTCTCGCTGGGATTCTTATGCTTACCAAAAAGC
TGAAGAAGACCGTCCGTGAAGATTTCGGCGCTTTTATCGATAAACTCATGCTTCTTCCCCCTCCGCAACCTGCTCCTCCCAAAGCTCCTCATCCTCTCAC
CGGTCTAACCTTTGCCGTTTCCGACGTGTGAGTCTCCGTTGACTTGTTTCCCCCTTTCTTCTCCCATATGTTTATCATATCTATATTTGGGGGTTTAGCT
GAATGATTTTTTCCTTGGGTAGAGTCAAAATTTTGTTTGACAAATGTTGGAATCTGCGTTGACTCGACTCGTAATCTTCTCCGGCGCACTACATGTCTGA
TT
Oligo 1
PRIMER_PAIR_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE 163
PRIMER_PAIR_PENALTY 0.1424
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 342 PRIMER_RIGHT_POSITION 504
PRIMER_LEFT_PENALTY 0.073866 PRIMER_RIGHT_PENALTY 0.068521
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_END_STABILITY 10.9000 PRIMER_RIGHT_END_STABILITY 8.8000
PRIMER_LEFT_TM 60.074 PRIMER_RIGHT_TM 59.931
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 6.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 110
PRIMER_PAIR_PENALTY_1 0.1622
PRIMER_PAIR_1_COMPL_END 3.00
PRIMER_PAIR_1_COMPL_ANY 6.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.074 PRIMER_RIGHT_1_TM 60.088
PRIMER_LEFT_1_END_STABILITY 10.9000 PRIMER_RIGHT_1_END_STABILITY 7.9000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_1_SEQUENCE GGGAAACAAGTCAACGGAGA
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 2.00
PRIMER_LEFT_1 342,20 PRIMER_RIGHT_1 451,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.073866 PRIMER_RIGHT_1_PENALTY 0.088296


Oligo 3
PRIMER_PRODUCT_SIZE_2 100
PRIMER_PAIR_2_COMPL_ANY 3.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.2023
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 10.9000 PRIMER_RIGHT_2_END_STABILITY 7.8000
PRIMER_LEFT_2_SEQUENCE AACTCATGCTTCTTCCCCCT PRIMER_RIGHT_2_SEQUENCE TCAACGGAGACTCACACGTC
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 3.00
PRIMER_LEFT_2 342,20 PRIMER_RIGHT_2 441,20
PRIMER_LEFT_2_TM 60.074 PRIMER_RIGHT_2_TM 59.872
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 4.00 PRIMER_RIGHT_2_SELF_ANY 5.00
PRIMER_LEFT_2_PENALTY 0.073866 PRIMER_RIGHT_2_PENALTY 0.128480


Oligo 4
PRIMER_PRODUCT_SIZE_3 203
PRIMER_PAIR_PENALTY_3 0.2246
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.156061 PRIMER_RIGHT_3_PENALTY 0.068521
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 6.7000 PRIMER_RIGHT_3_END_STABILITY 8.8000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 59.844 PRIMER_RIGHT_3_TM 59.931
PRIMER_LEFT_3_SELF_ANY 5.00 PRIMER_RIGHT_3_SELF_ANY 6.00
PRIMER_LEFT_3_SEQUENCE AAGAAGACCGTCCGTGAAGA PRIMER_RIGHT_3_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_3 302,20 PRIMER_RIGHT_3 504,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 204
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.2246
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE GAAGAAGACCGTCCGTGAAG PRIMER_RIGHT_4_SEQUENCE CATTCAGCTAAACCCCCAAA
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 59.844 PRIMER_RIGHT_4_TM 59.931
PRIMER_LEFT_4_PENALTY 0.156061 PRIMER_RIGHT_4_PENALTY 0.068521
PRIMER_LEFT_4 301,20 PRIMER_RIGHT_4 504,20
PRIMER_LEFT_4_END_STABILITY 7.0000 PRIMER_RIGHT_4_END_STABILITY 8.8000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 5.00 PRIMER_RIGHT_4_SELF_ANY 6.00