gene: At3g16620

insert: T-DNA_LB.SALK_088522

[Graphical View]
Sequence
TCACCATGGCTCTCTCCAATCTCCGAATAATTAACCTTTAAATTTTCTACTCCTCTCTCATTTCTACTTGTTTCTTCCAAATCACACACTTCATCATTGG
AAGCTAAAGGAAAATCCTCTTGCAACACATCGGTTTTGAGAGATTCAGGTTTTAGACCCTCCTGCGAACCAATTGCTTCCTCAAAGACCTCTTCTTCATT
ATCCTTCACTTCATCAGATCCCACCAACTCACTAATTCTACCATCCTCTGCAAGCTTCTTCTCATCCCCATATAACCTGGTTACAATCTCAGCCCCATCT
CCCATTTCTAGGATCACCCAAAATCACGAAACACTGCACCAACCAAATTTTGTATCCTCGTTACTTTTAAATCCACAAGACACTAAATTAAATGGGAGTG
AAACTAATATTTTCCAAATCGTAGGTATATTTTGCTCATAGGAATTCAGAAAATACAAAGAAGAATCTATTGATATTGAGGAGAAAAAGTGATGGCGAGA
ATCTAACCTGGTTAGAGAAGGCAAAAGTCTGGAAAGAAAAAAAAACAACTTCATGCGCTTTTTTTGTATTTGTATAAATCGCCTTTTACTCGCCTGCGAC
AG
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 184
PRIMER_PAIR_PENALTY 0.2458
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 40.000 PRIMER_RIGHT_GC_PERCENT 50.000
PRIMER_LEFT_POSITION 313 PRIMER_RIGHT_POSITION 496
PRIMER_LEFT_PENALTY 0.059151 PRIMER_RIGHT_PENALTY 0.186600
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE TCACCCAAAATCACGAAACA PRIMER_RIGHT_SEQUENCE CGCCATCACTTTTTCTCCTC
PRIMER_LEFT_END_STABILITY 7.0000 PRIMER_RIGHT_END_STABILITY 7.9000
PRIMER_LEFT_TM 59.941 PRIMER_RIGHT_TM 59.813
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 2.00 PRIMER_RIGHT_SELF_ANY 2.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 186
PRIMER_PAIR_PENALTY_1 0.2458
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.941 PRIMER_RIGHT_1_TM 59.813
PRIMER_LEFT_1_END_STABILITY 7.0000 PRIMER_RIGHT_1_END_STABILITY 7.9000
PRIMER_LEFT_1_GC_PERCENT 40.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE TCACCCAAAATCACGAAACA PRIMER_RIGHT_1_SEQUENCE CTCGCCATCACTTTTTCTCC
PRIMER_LEFT_1_SELF_ANY 2.00 PRIMER_RIGHT_1_SELF_ANY 2.00
PRIMER_LEFT_1 313,20 PRIMER_RIGHT_1 498,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 0.00
PRIMER_LEFT_1_PENALTY 0.059151 PRIMER_RIGHT_1_PENALTY 0.186600


Oligo 3
PRIMER_PRODUCT_SIZE_2 185
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 2.00
PRIMER_PAIR_PENALTY_2 0.2458
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 7.0000 PRIMER_RIGHT_2_END_STABILITY 7.9000
PRIMER_LEFT_2_SEQUENCE TCACCCAAAATCACGAAACA PRIMER_RIGHT_2_SEQUENCE TCGCCATCACTTTTTCTCCT
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 0.00
PRIMER_LEFT_2 313,20 PRIMER_RIGHT_2 497,20
PRIMER_LEFT_2_TM 59.941 PRIMER_RIGHT_2_TM 59.813
PRIMER_LEFT_2_GC_PERCENT 40.000 PRIMER_RIGHT_2_GC_PERCENT 45.000
PRIMER_LEFT_2_SELF_ANY 2.00 PRIMER_RIGHT_2_SELF_ANY 2.00
PRIMER_LEFT_2_PENALTY 0.059151 PRIMER_RIGHT_2_PENALTY 0.186600


Oligo 4
PRIMER_PRODUCT_SIZE_3 175
PRIMER_PAIR_PENALTY_3 0.3463
PRIMER_PAIR_3_COMPL_ANY 5.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.159749 PRIMER_RIGHT_3_PENALTY 0.186600
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 8.2000 PRIMER_RIGHT_3_END_STABILITY 7.9000
PRIMER_LEFT_3_GC_PERCENT 45.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 60.160 PRIMER_RIGHT_3_TM 59.813
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 2.00
PRIMER_LEFT_3_SEQUENCE ATCACGAAACACTGCACCAA PRIMER_RIGHT_3_SEQUENCE CGCCATCACTTTTTCTCCTC
PRIMER_LEFT_3 322,20 PRIMER_RIGHT_3 496,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE_4 176
PRIMER_PAIR_4_COMPL_END 1.00
PRIMER_PAIR_PENALTY_4 0.3463
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE ATCACGAAACACTGCACCAA PRIMER_RIGHT_4_SEQUENCE TCGCCATCACTTTTTCTCCT
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 60.160 PRIMER_RIGHT_4_TM 59.813
PRIMER_LEFT_4_PENALTY 0.159749 PRIMER_RIGHT_4_PENALTY 0.186600
PRIMER_LEFT_4 322,20 PRIMER_RIGHT_4 497,20
PRIMER_LEFT_4_END_STABILITY 8.2000 PRIMER_RIGHT_4_END_STABILITY 7.9000
PRIMER_LEFT_4_GC_PERCENT 45.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 2.00



insert: T-DNA_LB.SALK_099767

[Graphical View]
Sequence
GTTTTGACTTTCAGTCTCTCCCTTCTCCTCCGAACTAGATTTTTCCAATGGAGATAAGGTCCCAGCTGCTACTTCAATACTATCATTCAAGGATTTGTCA
CCTGAAACAATTTTAACACTATTGAACTCAGCACCAGTCTTACCATTTCTTTGTTCTGTTACCATGCCAGCTGATACATCAATTTCTTCCTCCCATTTTT
CGTCGTCAATACCATTACTCCCTGTTTCCACGGAACAGGCACTTACTGAGATAACTTCCGCCACCAGTTCTTTATTCTCATCAGGAGAGACTACTCCATT
TTCTGAAGAAGAATGTGTATTTCCGTTTTCCAAATTCACATCCTCAGTAGCTAAATTGGAAGTAGCTTTCTCACTGTTCTCAACCACATCCAAGCTTGAT
GCCATTTTACCATAAGAAATATCTGCATCTGCTACTTCCCCGTGATCATAATCATTCATTTTGAGAGTCACCAAATCAGAATCAGCCTCTTTCGTAGTTA
TACATTGCTCGTTAACCTCACCATGGCTCTCTCCAATCTCCGAATAATTAACCTTTAAATTTTCTACTCCTCTCTCATTTCTACTTGTTTCTTCCAAATC
AC
Oligo 1
PRIMER_PAIR_COMPL_ANY 7.00
PRIMER_PRODUCT_SIZE 117
PRIMER_PAIR_PENALTY 0.1484
PRIMER_PAIR_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 55.000 PRIMER_RIGHT_GC_PERCENT 50.000
PRIMER_LEFT_POSITION 56 PRIMER_RIGHT_POSITION 172
PRIMER_LEFT_PENALTY 0.012470 PRIMER_RIGHT_PENALTY 0.135900
PRIMER_LEFT_SELF_END 1.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE AGGTCCCAGCTGCTACTTCA PRIMER_RIGHT_SEQUENCE CAGCTGGCATGGTAACAGAA
PRIMER_LEFT_END_STABILITY 7.0000 PRIMER_RIGHT_END_STABILITY 7.0000
PRIMER_LEFT_TM 60.012 PRIMER_RIGHT_TM 59.864
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 6.00 PRIMER_RIGHT_SELF_ANY 6.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 191
PRIMER_PAIR_PENALTY_1 0.1833
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.012 PRIMER_RIGHT_1_TM 60.171
PRIMER_LEFT_1_END_STABILITY 7.0000 PRIMER_RIGHT_1_END_STABILITY 9.9000
PRIMER_LEFT_1_GC_PERCENT 55.000 PRIMER_RIGHT_1_GC_PERCENT 55.000
PRIMER_LEFT_1_SEQUENCE AGGTCCCAGCTGCTACTTCA PRIMER_RIGHT_1_SEQUENCE AGTAAGTGCCTGTTCCGTGG
PRIMER_LEFT_1_SELF_ANY 6.00 PRIMER_RIGHT_1_SELF_ANY 2.00
PRIMER_LEFT_1 56,20 PRIMER_RIGHT_1 246,20
PRIMER_LEFT_1_SELF_END 1.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.012470 PRIMER_RIGHT_1_PENALTY 0.170871


Oligo 3
PRIMER_PRODUCT_SIZE_2 161
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 2.00
PRIMER_PAIR_PENALTY_2 0.1907
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 9.9000 PRIMER_RIGHT_2_END_STABILITY 7.0000
PRIMER_LEFT_2_SEQUENCE AGTCTCTCCCTTCTCCTCCG PRIMER_RIGHT_2_SEQUENCE CAGCTGGCATGGTAACAGAA
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 0.00
PRIMER_LEFT_2 12,20 PRIMER_RIGHT_2 172,20
PRIMER_LEFT_2_TM 59.945 PRIMER_RIGHT_2_TM 59.864
PRIMER_LEFT_2_GC_PERCENT 60.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 3.00 PRIMER_RIGHT_2_SELF_ANY 6.00
PRIMER_LEFT_2_PENALTY 0.054785 PRIMER_RIGHT_2_PENALTY 0.135900


Oligo 4
PRIMER_PRODUCT_SIZE_3 235
PRIMER_PAIR_PENALTY_3 0.2257
PRIMER_PAIR_3_COMPL_ANY 3.00
PRIMER_PAIR_3_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.054785 PRIMER_RIGHT_3_PENALTY 0.170871
PRIMER_LEFT_3_SELF_END 2.00 PRIMER_RIGHT_3_SELF_END 2.00
PRIMER_LEFT_3_END_STABILITY 9.9000 PRIMER_RIGHT_3_END_STABILITY 9.9000
PRIMER_LEFT_3_GC_PERCENT 60.000 PRIMER_RIGHT_3_GC_PERCENT 55.000
PRIMER_LEFT_3_TM 59.945 PRIMER_RIGHT_3_TM 60.171
PRIMER_LEFT_3_SELF_ANY 3.00 PRIMER_RIGHT_3_SELF_ANY 2.00
PRIMER_LEFT_3_SEQUENCE AGTCTCTCCCTTCTCCTCCG PRIMER_RIGHT_3_SEQUENCE AGTAAGTGCCTGTTCCGTGG
PRIMER_LEFT_3 12,20 PRIMER_RIGHT_3 246,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 218
PRIMER_PAIR_4_COMPL_END 3.00
PRIMER_PAIR_PENALTY_4 0.2535
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE AGGTCCCAGCTGCTACTTCA PRIMER_RIGHT_4_SEQUENCE TAAAGAACTGGTGGCGGAAG
PRIMER_LEFT_4_SELF_END 1.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 60.012 PRIMER_RIGHT_4_TM 60.241
PRIMER_LEFT_4_PENALTY 0.012470 PRIMER_RIGHT_4_PENALTY 0.241032
PRIMER_LEFT_4 56,20 PRIMER_RIGHT_4 273,20
PRIMER_LEFT_4_END_STABILITY 7.0000 PRIMER_RIGHT_4_END_STABILITY 8.2000
PRIMER_LEFT_4_GC_PERCENT 55.000 PRIMER_RIGHT_4_GC_PERCENT 50.000
PRIMER_LEFT_4_SELF_ANY 6.00 PRIMER_RIGHT_4_SELF_ANY 2.00



insert: T-DNA_LB.SALK_043456

[Graphical View]
Sequence
TGGTTGCACTTTTACCAACCCCACTTTTACCAAGCACCATAATCGTACAAGAAAAATCAAGGGGATCTTGTGCAGCTGCCTCAAGCTGTTCTGCCATGGC
ACTGGCACGATCAAAACTAAAGGCACCAACACGGCTTCCGTTTCTGCCCCTCAACTGTTCAGCCAATCCAAGCCTGTACAAAACCTGAGCAACAACAACA
TTATGTGGAGTTTGCCCTAATCTATGTGAAAGCCTCAAAAATTTGACCCTGATAAACTGGAGCTTCTCACGGGTCTCATCATGCTCATCTGTCTCTGCAG
TGGTAGAGTCTTCAGCTTGCTGAGGCTGATTGTGAGACACATTTCCATTGACACGAGGCTGTTGTGTAACGCGTGGTGCAGGTTCCAAAAGTGGAGCAGC
ACGACCAAGACCTGCTGGGCGAGCAGGTGGTAATGCTGCAGTATCACTTCTAGACTCTGTTGGTGAAACAGAACTTAATACCTCAGATTCTCTTTCCATA
TGTTGGCTTTCCTTAATCTCTGGACCTATATTTACACTGCTATCTTGCTGCTTTACAATTTCCTTATTAGATTGAATATCATGTCCTCCATTACTGTTTT
GA
Oligo 1
PRIMER_PAIR_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE 278
PRIMER_PAIR_PENALTY 0.0427
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 50.000
PRIMER_LEFT_POSITION 114 PRIMER_RIGHT_POSITION 391
PRIMER_LEFT_PENALTY 0.030276 PRIMER_RIGHT_PENALTY 0.012470
PRIMER_LEFT_SELF_END 1.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE AACTAAAGGCACCAACACGG PRIMER_RIGHT_SEQUENCE ACTTTTGGAACCTGCACCAC
PRIMER_LEFT_END_STABILITY 9.9000 PRIMER_RIGHT_END_STABILITY 7.6000
PRIMER_LEFT_TM 60.030 PRIMER_RIGHT_TM 60.012
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 2.00 PRIMER_RIGHT_SELF_ANY 4.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 245
PRIMER_PAIR_PENALTY_1 0.1235
PRIMER_PAIR_1_COMPL_END 0.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.111 PRIMER_RIGHT_1_TM 60.012
PRIMER_LEFT_1_END_STABILITY 8.4000 PRIMER_RIGHT_1_END_STABILITY 7.6000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE CCCTCAACTGTTCAGCCAAT PRIMER_RIGHT_1_SEQUENCE ACTTTTGGAACCTGCACCAC
PRIMER_LEFT_1_SELF_ANY 7.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 147,20 PRIMER_RIGHT_1 391,20
PRIMER_LEFT_1_SELF_END 2.00 PRIMER_RIGHT_1_SELF_END 0.00
PRIMER_LEFT_1_PENALTY 0.111077 PRIMER_RIGHT_1_PENALTY 0.012470


Oligo 3
PRIMER_PRODUCT_SIZE_2 212
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.1327
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 9.9000 PRIMER_RIGHT_2_END_STABILITY 6.1000
PRIMER_LEFT_2_SEQUENCE AACTAAAGGCACCAACACGG PRIMER_RIGHT_2_SEQUENCE GCCTCAGCAAGCTGAAGACT
PRIMER_LEFT_2_SELF_END 1.00 PRIMER_RIGHT_2_SELF_END 3.00
PRIMER_LEFT_2 114,20 PRIMER_RIGHT_2 325,20
PRIMER_LEFT_2_TM 60.030 PRIMER_RIGHT_2_TM 59.898
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 55.000
PRIMER_LEFT_2_SELF_ANY 2.00 PRIMER_RIGHT_2_SELF_ANY 8.00
PRIMER_LEFT_2_PENALTY 0.030276 PRIMER_RIGHT_2_PENALTY 0.102380


Oligo 4
PRIMER_PRODUCT_SIZE_3 221
PRIMER_PAIR_PENALTY_3 0.1663
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 0.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.030276 PRIMER_RIGHT_3_PENALTY 0.136001
PRIMER_LEFT_3_SELF_END 1.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 9.9000 PRIMER_RIGHT_3_END_STABILITY 8.5000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 60.030 PRIMER_RIGHT_3_TM 60.136
PRIMER_LEFT_3_SELF_ANY 2.00 PRIMER_RIGHT_3_SELF_ANY 3.00
PRIMER_LEFT_3_SEQUENCE AACTAAAGGCACCAACACGG PRIMER_RIGHT_3_SEQUENCE TCACAATCAGCCTCAGCAAG
PRIMER_LEFT_3 114,20 PRIMER_RIGHT_3 334,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 222
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.1663
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE AACTAAAGGCACCAACACGG PRIMER_RIGHT_4_SEQUENCE CTCACAATCAGCCTCAGCAA
PRIMER_LEFT_4_SELF_END 1.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 60.030 PRIMER_RIGHT_4_TM 60.136
PRIMER_LEFT_4_PENALTY 0.030276 PRIMER_RIGHT_4_PENALTY 0.136001
PRIMER_LEFT_4 114,20 PRIMER_RIGHT_4 335,20
PRIMER_LEFT_4_END_STABILITY 9.9000 PRIMER_RIGHT_4_END_STABILITY 8.5000
PRIMER_LEFT_4_GC_PERCENT 50.000 PRIMER_RIGHT_4_GC_PERCENT 50.000
PRIMER_LEFT_4_SELF_ANY 2.00 PRIMER_RIGHT_4_SELF_ANY 3.00



insert: T-DNA_LB.SALK_017374.51.20.x

[Graphical View]
Sequence
CTAGGGAAGTTGATCTACCTTCTCCATGTTTAACCGAGCTGGCCAATTCTAGCTGCACATGTGCATCCTTCTTGTCCTTTGTCACTTGGCCAGAGAAAGA
TACTGGTATTTTGTCTTTAACAACAAATAGTCGTTCCGCATTCACACCTTCATAACCAATATCATGATCCCACCCATGAGTTTCCAGCACTGGCCTAACA
AGCCATTGATTGGAGGTATCAAGGTACCGGTACCGATGAGTAGGATTGTCAGAGTCAAAAGATGCAGGTAGAGATAAATCTGGCATTGGAACTGGAACAG
ATGCAGGTTCACTTCTCTCCTCTTCCACATTTTCACTATACCCGTTAGGCATATCTTTAATCTCAGCAGCAAATTTCTTCAACAACTTACGTCTCTTTCT
TTCCTCTTTCATCTGTCTCTTCATAAATAGTTTCTCCCGATACTCCATCTCATCGAGATATTCCTTCTTCTGAGATTTGCTAAGCTTAGTCATCTCTGCT
TTAGTCAACCGCTTAAAGGGAGGAAGCTCATCATACTCTGATTCTTCCTCAGAATCTGATGATTCGTCTAAATCATCTTCATCGTCTTCATCATCGTACT
GT
Oligo 1
PRIMER_PAIR_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE 161
PRIMER_PAIR_PENALTY 0.1401
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 45.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 134 PRIMER_RIGHT_POSITION 294
PRIMER_LEFT_PENALTY 0.072672 PRIMER_RIGHT_PENALTY 0.067436
PRIMER_LEFT_SELF_END 2.00 PRIMER_RIGHT_SELF_END 2.00
PRIMER_LEFT_SEQUENCE TCCGCATTCACACCTTCATA PRIMER_RIGHT_SEQUENCE CCAGTTCCAATGCCAGATTT
PRIMER_LEFT_END_STABILITY 5.9000 PRIMER_RIGHT_END_STABILITY 6.9000
PRIMER_LEFT_TM 60.073 PRIMER_RIGHT_TM 59.933
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 2.00 PRIMER_RIGHT_SELF_ANY 3.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 181
PRIMER_PAIR_PENALTY_1 0.1853
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.118 PRIMER_RIGHT_1_TM 59.933
PRIMER_LEFT_1_END_STABILITY 8.2000 PRIMER_RIGHT_1_END_STABILITY 7.0000
PRIMER_LEFT_1_GC_PERCENT 55.000 PRIMER_RIGHT_1_GC_PERCENT 45.000
PRIMER_LEFT_1_SEQUENCE GAGCTGGCCAATTCTAGCTG PRIMER_RIGHT_1_SEQUENCE CCTCCAATCAATGGCTTGTT
PRIMER_LEFT_1_SELF_ANY 6.00 PRIMER_RIGHT_1_SELF_ANY 6.00
PRIMER_LEFT_1 35,20 PRIMER_RIGHT_1 215,20
PRIMER_LEFT_1_SELF_END 2.00 PRIMER_RIGHT_1_SELF_END 3.00
PRIMER_LEFT_1_PENALTY 0.117817 PRIMER_RIGHT_1_PENALTY 0.067436


Oligo 3
PRIMER_PRODUCT_SIZE_2 119
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 0.00
PRIMER_PAIR_PENALTY_2 0.1905
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 8.2000 PRIMER_RIGHT_2_END_STABILITY 11.4000
PRIMER_LEFT_2_SEQUENCE GAGCTGGCCAATTCTAGCTG PRIMER_RIGHT_2_SEQUENCE TATGAAGGTGTGAATGCGGA
PRIMER_LEFT_2_SELF_END 2.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 35,20 PRIMER_RIGHT_2 153,20
PRIMER_LEFT_2_TM 60.118 PRIMER_RIGHT_2_TM 60.073
PRIMER_LEFT_2_GC_PERCENT 55.000 PRIMER_RIGHT_2_GC_PERCENT 45.000
PRIMER_LEFT_2_SELF_ANY 6.00 PRIMER_RIGHT_2_SELF_ANY 2.00
PRIMER_LEFT_2_PENALTY 0.117817 PRIMER_RIGHT_2_PENALTY 0.072672


Oligo 4
PRIMER_PRODUCT_SIZE_3 117
PRIMER_PAIR_PENALTY_3 0.1921
PRIMER_PAIR_3_COMPL_ANY 5.00
PRIMER_PAIR_3_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.124655 PRIMER_RIGHT_3_PENALTY 0.067436
PRIMER_LEFT_3_SELF_END 1.00 PRIMER_RIGHT_3_SELF_END 2.00
PRIMER_LEFT_3_END_STABILITY 7.5000 PRIMER_RIGHT_3_END_STABILITY 6.9000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 45.000
PRIMER_LEFT_3_TM 59.875 PRIMER_RIGHT_3_TM 59.933
PRIMER_LEFT_3_SELF_ANY 6.00 PRIMER_RIGHT_3_SELF_ANY 3.00
PRIMER_LEFT_3_SEQUENCE AGTTTCCAGCACTGGCCTAA PRIMER_RIGHT_3_SEQUENCE CCAGTTCCAATGCCAGATTT
PRIMER_LEFT_3 178,20 PRIMER_RIGHT_3 294,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE_4 107
PRIMER_PAIR_4_COMPL_END 3.00
PRIMER_PAIR_PENALTY_4 0.1997
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE ACTGGCCTAACAAGCCATTG PRIMER_RIGHT_4_SEQUENCE CCAGTTCCAATGCCAGATTT
PRIMER_LEFT_4_SELF_END 3.00 PRIMER_RIGHT_4_SELF_END 2.00
PRIMER_LEFT_4_TM 60.132 PRIMER_RIGHT_4_TM 59.933
PRIMER_LEFT_4_PENALTY 0.132262 PRIMER_RIGHT_4_PENALTY 0.067436
PRIMER_LEFT_4 188,20 PRIMER_RIGHT_4 294,20
PRIMER_LEFT_4_END_STABILITY 7.2000 PRIMER_RIGHT_4_END_STABILITY 6.9000
PRIMER_LEFT_4_GC_PERCENT 50.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 5.00 PRIMER_RIGHT_4_SELF_ANY 3.00