gene: At2g11810

insert: T-DNA_LB.SALK_066984

[Graphical View]
Sequence
TACCTCGAATAATATGTATTTATATTATGACAAAATTTTCCAATAGATTATGTTATAATATATCATAGAGGAGTTTCTGATTCTAATGAAATCTCAGCCG
TCAATATTAAAGTTGTCGCAGTAGTCGTCTTGAATTTGAAAACGAAAAGAAACAAATATCTACTTGGAAGTTTTGTAGCCTAGTATGAATCTTGGAGTTT
GCCAGGGTGTCGGAGATACGAGCTGACGTCAACAATCTATGTCTTAAAACCTGTCCCACGTTCCATTTATTTTGTTTGTCAATAATGATTAAAAATTTGC
ATTATTTATTTCTCTAGCTCTGACTCTTTGATGTTACAATATTCTCTCTAGGAACTAATGTCATTTTTTTTATAACTATAGATAATCATAAAAGATGTTT
GGAAAGAATACACTGGATGGCCATTGAACGACATGGAGAGACAGTACAAGTTCATGGTGAAACATGTTGGTCTTTGGTCTGTTGCGTTTCATGGTACTTC
TCCCAAATGGATCCACAAAAGCTATCTAAGTGCTCTTGCCGCTTATTATGCCAAGTACTTATATGTTAACCTTCTTCTTTTTTCGTTATCATCCCTTAGT
GT
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 247
PRIMER_PAIR_PENALTY 0.2891
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 244 PRIMER_RIGHT_POSITION 490
PRIMER_LEFT_PENALTY 0.170049 PRIMER_RIGHT_PENALTY 0.119028
PRIMER_LEFT_SELF_END 1.00 PRIMER_RIGHT_SELF_END 0.00
PRIMER_LEFT_SEQUENCE TAAAACCTGTCCCACGTTCC PRIMER_RIGHT_SEQUENCE TGAAACGCAACAGACCAAAG
PRIMER_LEFT_END_STABILITY 7.9000 PRIMER_RIGHT_END_STABILITY 7.3000
PRIMER_LEFT_TM 59.830 PRIMER_RIGHT_TM 59.881
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 5.00 PRIMER_RIGHT_SELF_ANY 2.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 244
PRIMER_PAIR_PENALTY_1 0.3263
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.830 PRIMER_RIGHT_1_TM 60.156
PRIMER_LEFT_1_END_STABILITY 7.9000 PRIMER_RIGHT_1_END_STABILITY 7.6000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE TAAAACCTGTCCCACGTTCC PRIMER_RIGHT_1_SEQUENCE AACGCAACAGACCAAAGACC
PRIMER_LEFT_1_SELF_ANY 5.00 PRIMER_RIGHT_1_SELF_ANY 2.00
PRIMER_LEFT_1 244,20 PRIMER_RIGHT_1 487,20
PRIMER_LEFT_1_SELF_END 1.00 PRIMER_RIGHT_1_SELF_END 0.00
PRIMER_LEFT_1_PENALTY 0.170049 PRIMER_RIGHT_1_PENALTY 0.156287


Oligo 3
PRIMER_PRODUCT_SIZE_2 277
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 2.00
PRIMER_PAIR_PENALTY_2 0.3321
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 7.9000 PRIMER_RIGHT_2_END_STABILITY 10.0000
PRIMER_LEFT_2_SEQUENCE TAAAACCTGTCCCACGTTCC PRIMER_RIGHT_2_SEQUENCE CTTTTGTGGATCCATTTGGG
PRIMER_LEFT_2_SELF_END 1.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 244,20 PRIMER_RIGHT_2 520,20
PRIMER_LEFT_2_TM 59.830 PRIMER_RIGHT_2_TM 60.162
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 45.000
PRIMER_LEFT_2_SELF_ANY 5.00 PRIMER_RIGHT_2_SELF_ANY 8.00
PRIMER_LEFT_2_PENALTY 0.170049 PRIMER_RIGHT_2_PENALTY 0.162074


Oligo 4
PRIMER_PRODUCT_SIZE_3 300
PRIMER_PAIR_PENALTY_3 0.4617
PRIMER_PAIR_3_COMPL_ANY 3.00
PRIMER_PAIR_3_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.170049 PRIMER_RIGHT_3_PENALTY 0.291669
PRIMER_LEFT_3_SELF_END 1.00 PRIMER_RIGHT_3_SELF_END 2.00
PRIMER_LEFT_3_END_STABILITY 7.9000 PRIMER_RIGHT_3_END_STABILITY 6.0000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 50.000
PRIMER_LEFT_3_TM 59.830 PRIMER_RIGHT_3_TM 60.292
PRIMER_LEFT_3_SELF_ANY 5.00 PRIMER_RIGHT_3_SELF_ANY 6.00
PRIMER_LEFT_3_SEQUENCE TAAAACCTGTCCCACGTTCC PRIMER_RIGHT_3_SEQUENCE AAGCGGCAAGAGCACTTAGA
PRIMER_LEFT_3 244,20 PRIMER_RIGHT_3 543,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 3.00
PRIMER_PRODUCT_SIZE_4 289
PRIMER_PAIR_4_COMPL_END 0.00
PRIMER_PAIR_PENALTY_4 0.7871
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE CAGGGTGTCGGAGATACGAG PRIMER_RIGHT_4_SEQUENCE TGAAACGCAACAGACCAAAG
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 0.00
PRIMER_LEFT_4_TM 60.668 PRIMER_RIGHT_4_TM 59.881
PRIMER_LEFT_4_PENALTY 0.668065 PRIMER_RIGHT_4_PENALTY 0.119028
PRIMER_LEFT_4 202,20 PRIMER_RIGHT_4 490,20
PRIMER_LEFT_4_END_STABILITY 8.1000 PRIMER_RIGHT_4_END_STABILITY 7.3000
PRIMER_LEFT_4_GC_PERCENT 60.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 2.00



insert: T-DNA_LB.SALK_069971.51.75.x

[Graphical View]
Sequence
CATTCTCTTTGCCTTGAATCCAACGCATAGGGTTGAGCCAATAATATGCTTCCTTAACCATATATATAACGTACAAAAAAACGAAAAAATATTGCATAAG
CAACAAAACTTAATCATACTTTTATCTTTTAGTTTCTGTGTAAAACAACAACAATATTTTGAAATATTCATTTTGAAAATTTCAAAGCTTGTAAATATAT
ACCTCGAATAATATGTATTTATATTATGACAAAATTTTCCAATAGATTATGTTATAATATATCATAGAGGAGTTTCTGATTCTAATGAAATCTCAGCCGT
CAATATTAAAGTTGTCGCAGTAGTCGTCTTGAATTTGAAAACGAAAAGAAACAAATATCTACTTGGAAGTTTTGTAGCCTAGTATGAATCTTGGAGTTTG
CCAGGGTGTCGGAGATACGAGCTGACGTCAACAATCTATGTCTTAAAACCTGTCCCACGTTCCATTTATTTTGTTTGTCAATAATGATTAAAAATTTGCA
TTATTTATTTCTCTAGCTCTGACTCTTTGATGTTACAATATTCTCTCTAGGAACTAATGTCATTTTTTTTATAACTATAGATAATCATAAAAGATGTTTG
GA
Oligo 1
PRIMER_PAIR_COMPL_ANY 5.00
PRIMER_PRODUCT_SIZE 152
PRIMER_PAIR_PENALTY 1.5212
PRIMER_PAIR_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 47.619 PRIMER_RIGHT_GC_PERCENT 50.000
PRIMER_LEFT_POSITION 311 PRIMER_RIGHT_POSITION 462
PRIMER_LEFT_PENALTY 1.351148 PRIMER_RIGHT_PENALTY 0.170049
PRIMER_LEFT_SELF_END 2.00 PRIMER_RIGHT_SELF_END 2.00
PRIMER_LEFT_SEQUENCE TTGTCGCAGTAGTCGTCTTGA PRIMER_RIGHT_SEQUENCE GGAACGTGGGACAGGTTTTA
PRIMER_LEFT_END_STABILITY 7.0000 PRIMER_RIGHT_END_STABILITY 6.6000
PRIMER_LEFT_TM 59.649 PRIMER_RIGHT_TM 59.830
PRIMER_LEFT_LENGTH 21 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 4.00 PRIMER_RIGHT_SELF_ANY 5.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 151
PRIMER_PAIR_PENALTY_1 1.5212
PRIMER_PAIR_1_COMPL_END 2.00
PRIMER_PAIR_1_COMPL_ANY 5.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 59.649 PRIMER_RIGHT_1_TM 59.830
PRIMER_LEFT_1_END_STABILITY 7.3000 PRIMER_RIGHT_1_END_STABILITY 6.6000
PRIMER_LEFT_1_GC_PERCENT 47.619 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE TGTCGCAGTAGTCGTCTTGAA PRIMER_RIGHT_1_SEQUENCE GGAACGTGGGACAGGTTTTA
PRIMER_LEFT_1_SELF_ANY 4.00 PRIMER_RIGHT_1_SELF_ANY 5.00
PRIMER_LEFT_1 312,21 PRIMER_RIGHT_1 462,20
PRIMER_LEFT_1_SELF_END 3.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 1.351148 PRIMER_RIGHT_1_PENALTY 0.170049


Oligo 3
PRIMER_PRODUCT_SIZE_2 180
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 2.00
PRIMER_PAIR_PENALTY_2 1.8719
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 6.7000 PRIMER_RIGHT_2_END_STABILITY 6.6000
PRIMER_LEFT_2_SEQUENCE AATGAAATCTCAGCCGTCAA PRIMER_RIGHT_2_SEQUENCE GGAACGTGGGACAGGTTTTA
PRIMER_LEFT_2_SELF_END 1.00 PRIMER_RIGHT_2_SELF_END 2.00
PRIMER_LEFT_2 283,20 PRIMER_RIGHT_2 462,20
PRIMER_LEFT_2_TM 58.298 PRIMER_RIGHT_2_TM 59.830
PRIMER_LEFT_2_GC_PERCENT 40.000 PRIMER_RIGHT_2_GC_PERCENT 50.000
PRIMER_LEFT_2_SELF_ANY 6.00 PRIMER_RIGHT_2_SELF_ANY 5.00
PRIMER_LEFT_2_PENALTY 1.701882 PRIMER_RIGHT_2_PENALTY 0.170049


Oligo 4
PRIMER_PRODUCT_SIZE_3 109
PRIMER_PAIR_PENALTY_3 2.0192
PRIMER_PAIR_3_COMPL_ANY 6.00
PRIMER_PAIR_3_COMPL_END 1.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 1.351148 PRIMER_RIGHT_3_PENALTY 0.668065
PRIMER_LEFT_3_SELF_END 3.00 PRIMER_RIGHT_3_SELF_END 1.00
PRIMER_LEFT_3_END_STABILITY 7.3000 PRIMER_RIGHT_3_END_STABILITY 9.7000
PRIMER_LEFT_3_GC_PERCENT 47.619 PRIMER_RIGHT_3_GC_PERCENT 60.000
PRIMER_LEFT_3_TM 59.649 PRIMER_RIGHT_3_TM 60.668
PRIMER_LEFT_3_SELF_ANY 4.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE TGTCGCAGTAGTCGTCTTGAA PRIMER_RIGHT_3_SEQUENCE CTCGTATCTCCGACACCCTG
PRIMER_LEFT_3 312,21 PRIMER_RIGHT_3 420,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 6.00
PRIMER_PRODUCT_SIZE_4 110
PRIMER_PAIR_4_COMPL_END 2.00
PRIMER_PAIR_PENALTY_4 2.0192
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE TTGTCGCAGTAGTCGTCTTGA PRIMER_RIGHT_4_SEQUENCE CTCGTATCTCCGACACCCTG
PRIMER_LEFT_4_SELF_END 2.00 PRIMER_RIGHT_4_SELF_END 1.00
PRIMER_LEFT_4_TM 59.649 PRIMER_RIGHT_4_TM 60.668
PRIMER_LEFT_4_PENALTY 1.351148 PRIMER_RIGHT_4_PENALTY 0.668065
PRIMER_LEFT_4 311,21 PRIMER_RIGHT_4 420,20
PRIMER_LEFT_4_END_STABILITY 7.0000 PRIMER_RIGHT_4_END_STABILITY 9.7000
PRIMER_LEFT_4_GC_PERCENT 47.619 PRIMER_RIGHT_4_GC_PERCENT 60.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 4.00



insert: T-DNA_LB.SALK_084186.29.90.x

[Graphical View]
Sequence
TCACCAACCAGCCACATAGAAAACACAACCATCGGTCTTCTTATTATTCTTTCTTCAACACCAAATAGTCATGATGAAAGTGGTGTCACCTCGTACACGG
TCGGATTCAATCACTGAGAAGGTGTTTCGACGAGTCTATAGCAATTTTAACATCTCAACAGTAGAAGATGAGTATATCCATCGTCAGAGATCAAGTGATT
ATGAGAAGGAGAGTCGTCTAAGGAAGAGAGGGTTAGAAGACAAAGAAGAAGTTATGGAGATGGAGCAGATGGGAGCAGAGAGGATCAAAACTGTTCTTAT
TCTCATGAGTGATACCGGCGGTGGCCACCGTGCTTCAGCCGAGGCCATCCGCGACGCTTTCAAGATCGAATTCGGAGATGACTATCGGGTAAAAATATTA
ATATTTTTATTTATATCTTCATTTTCATTCTCTTTGCCTTGAATCCAACGCATAGGGTTGAGCCAATAATATGCTTCCTTAACCATATATATAACGTACA
AAAAAACGAAAAAATATTGCATAAGCAACAAAACTTAATCATACTTTTATCTTTTAGTTTCTGTGTAAAACAACAACAATATTTTGAAATATTCATTTTG
AA
Oligo 1
PRIMER_PAIR_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE 113
PRIMER_PAIR_PENALTY 0.1879
PRIMER_PAIR_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_GC_PERCENT 50.000 PRIMER_RIGHT_GC_PERCENT 45.000
PRIMER_LEFT_POSITION 0 PRIMER_RIGHT_POSITION 112
PRIMER_LEFT_PENALTY 0.112124 PRIMER_RIGHT_PENALTY 0.075792
PRIMER_LEFT_SELF_END 0.00 PRIMER_RIGHT_SELF_END 2.00
PRIMER_LEFT_SEQUENCE TCACCAACCAGCCACATAGA PRIMER_RIGHT_SEQUENCE TGATTGAATCCGACCGTGTA
PRIMER_LEFT_END_STABILITY 5.6000 PRIMER_RIGHT_END_STABILITY 5.4000
PRIMER_LEFT_TM 60.112 PRIMER_RIGHT_TM 59.924
PRIMER_LEFT_LENGTH 20 PRIMER_RIGHT_LENGTH 20
PRIMER_LEFT_SELF_ANY 2.00 PRIMER_RIGHT_SELF_ANY 7.00


Oligo 2
PRIMER_PRODUCT_SIZE_1 288
PRIMER_PAIR_PENALTY_1 0.3506
PRIMER_PAIR_1_COMPL_END 1.00
PRIMER_PAIR_1_COMPL_ANY 4.00
Left Primer Right Primer
PRIMER_LEFT_1_TM 60.112 PRIMER_RIGHT_1_TM 59.761
PRIMER_LEFT_1_END_STABILITY 5.6000 PRIMER_RIGHT_1_END_STABILITY 9.6000
PRIMER_LEFT_1_GC_PERCENT 50.000 PRIMER_RIGHT_1_GC_PERCENT 50.000
PRIMER_LEFT_1_SEQUENCE TCACCAACCAGCCACATAGA PRIMER_RIGHT_1_SEQUENCE TTGATCCTCTCTGCTCCCAT
PRIMER_LEFT_1_SELF_ANY 2.00 PRIMER_RIGHT_1_SELF_ANY 4.00
PRIMER_LEFT_1 0,20 PRIMER_RIGHT_1 287,20
PRIMER_LEFT_1_SELF_END 0.00 PRIMER_RIGHT_1_SELF_END 2.00
PRIMER_LEFT_1_PENALTY 0.112124 PRIMER_RIGHT_1_PENALTY 0.238514


Oligo 3
PRIMER_PRODUCT_SIZE_2 282
PRIMER_PAIR_2_COMPL_ANY 4.00
PRIMER_PAIR_2_COMPL_END 2.00
PRIMER_PAIR_PENALTY_2 0.3582
Left Primer Right Primer
PRIMER_LEFT_2_END_STABILITY 5.6000 PRIMER_RIGHT_2_END_STABILITY 8.2000
PRIMER_LEFT_2_SEQUENCE TCACCAACCAGCCACATAGA PRIMER_RIGHT_2_SEQUENCE CTCTCTGCTCCCATCTGCTC
PRIMER_LEFT_2_SELF_END 0.00 PRIMER_RIGHT_2_SELF_END 0.00
PRIMER_LEFT_2 0,20 PRIMER_RIGHT_2 281,20
PRIMER_LEFT_2_TM 60.112 PRIMER_RIGHT_2_TM 60.246
PRIMER_LEFT_2_GC_PERCENT 50.000 PRIMER_RIGHT_2_GC_PERCENT 60.000
PRIMER_LEFT_2_SELF_ANY 2.00 PRIMER_RIGHT_2_SELF_ANY 2.00
PRIMER_LEFT_2_PENALTY 0.112124 PRIMER_RIGHT_2_PENALTY 0.246121


Oligo 4
PRIMER_PRODUCT_SIZE_3 287
PRIMER_PAIR_PENALTY_3 0.4237
PRIMER_PAIR_3_COMPL_ANY 4.00
PRIMER_PAIR_3_COMPL_END 2.00
Left Primer Right Primer
PRIMER_LEFT_3_PENALTY 0.112124 PRIMER_RIGHT_3_PENALTY 0.311617
PRIMER_LEFT_3_SELF_END 0.00 PRIMER_RIGHT_3_SELF_END 0.00
PRIMER_LEFT_3_END_STABILITY 5.6000 PRIMER_RIGHT_3_END_STABILITY 8.1000
PRIMER_LEFT_3_GC_PERCENT 50.000 PRIMER_RIGHT_3_GC_PERCENT 55.000
PRIMER_LEFT_3_TM 60.112 PRIMER_RIGHT_3_TM 60.312
PRIMER_LEFT_3_SELF_ANY 2.00 PRIMER_RIGHT_3_SELF_ANY 4.00
PRIMER_LEFT_3_SEQUENCE TCACCAACCAGCCACATAGA PRIMER_RIGHT_3_SEQUENCE TGATCCTCTCTGCTCCCATC
PRIMER_LEFT_3 0,20 PRIMER_RIGHT_3 286,20


Oligo 5
PRIMER_PAIR_4_COMPL_ANY 4.00
PRIMER_PRODUCT_SIZE_4 107
PRIMER_PAIR_4_COMPL_END 2.00
PRIMER_PAIR_PENALTY_4 0.4541
Left Primer Right Primer
PRIMER_LEFT_4_SEQUENCE ATGGGAGCAGAGAGGATCAA PRIMER_RIGHT_4_SEQUENCE CCGAATTCGATCTTGAAAGC
PRIMER_LEFT_4_SELF_END 0.00 PRIMER_RIGHT_4_SELF_END 2.00
PRIMER_LEFT_4_TM 59.761 PRIMER_RIGHT_4_TM 59.784
PRIMER_LEFT_4_PENALTY 0.238514 PRIMER_RIGHT_4_PENALTY 0.215550
PRIMER_LEFT_4 268,20 PRIMER_RIGHT_4 374,20
PRIMER_LEFT_4_END_STABILITY 6.9000 PRIMER_RIGHT_4_END_STABILITY 8.5000
PRIMER_LEFT_4_GC_PERCENT 50.000 PRIMER_RIGHT_4_GC_PERCENT 45.000
PRIMER_LEFT_4_SELF_ANY 4.00 PRIMER_RIGHT_4_SELF_ANY 8.00